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show all sequences of 1.14.11.1

Comparison of the substrate selectivity and biochemical properties of human and bacterial gamma-butyrobetaine hydroxylase

Rydzik, A.M.; Leung, I.K.; Kochan, G.T.; Loik, N.D.; Henry, L.; McDonough, M.A.; Claridge, T.D.; Schofield, C.J.; Org. Biomol. Chem. 12, 6354-6358 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Homo sapiens
Application
Application
Commentary
Organism
medicine
BBOX is a therapeutic target in humans, the BBOX inhibitor mildronate is used to decrease fatty acid oxidation in patients after myocardial infarction
Homo sapiens
Cloned(Commentary)
Commentary
Organism
gene BBOX1, recombinant expression of His-tagged codon-optimized enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
recombinant expression of N-terminally His-tagged codon-optimized enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas sp.
Crystallization (Commentary)
Crystallization
Organism
crystal structure analysis, PDB ID 3O2G
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
(3R)-3-fluoro-4-(trimethylammonio)butanoate
-
Homo sapiens
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium
-
Homo sapiens
4-Trimethylammoniobutanoate
substrate inhibition
Homo sapiens
4-Trimethylammoniobutanoate
substrate inhibition at high concentrations
Pseudomonas sp.
mildronate
-
Homo sapiens
N-oxalylglycine
-
Homo sapiens
N-oxalylglycine
binding structure modeling
Pseudomonas sp.
Ni2+
binding structure modeling
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
additional information
-
additional information
Michaelis-Menten kinetics
Pseudomonas sp.
0.0042
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.02
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
0.02
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.153
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.163
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication, only the Michaelis-Menten range is fitted
Pseudomonas sp.
0.412
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.532
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.623
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
2.474
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication, the whole range is fitted
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
nonheme Fe2+-dependent oxygenase, dependent on, required for catalysis
Homo sapiens
Fe2+
nonheme Fe2+-dependent oxygenase, dependent on, required for catalysis
Pseudomonas sp.
additional information
Ni2+ cannot substitute for Fe2+
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Homo sapiens
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Pseudomonas sp.
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Pseudomonas sp. AK-1
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
O75936
-
-
Pseudomonas sp.
P80193
-
-
Pseudomonas sp. AK-1
P80193
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography
Homo sapiens
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography, to over 90% purity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(R)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
N-[(3R)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(R)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
N-[(3R)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
N-[(3S)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
N-[(3S)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-3-carboxy-2-hydroxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
(3S)-3-carboxy-2,3-dihydroxy-N,N,N-trimethylpropan-1-aminium + succinate + CO2
-
-
-
?
2-carboxy-N,N,N-trimethylethan-1-aminium + 2-oxoglutarate + O2
-
745953
Homo sapiens
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
2-carboxy-N,N,N-trimethylethan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Homo sapiens
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium + succinate + CO2
-
-
-
?
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniopropanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
2-carboxy-2-hydroxy-N,N,N-trimethylethan-1-aminium + succinate + CO2
-
-
-
?
D-carnitine + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
D-carnitine + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
L-carnitine + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Homo sapiens
?
-
-
-
-
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Pseudomonas sp.
?
-
-
-
-
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Pseudomonas sp. AK-1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
homology structure modelling
Homo sapiens
More
homology structure modelling
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.059
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.14
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.3
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
0.83
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
1.6
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Homo sapiens
3.5
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
5
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
21.3
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
7.5
-
assay at
Pseudomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.024
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.125
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.136
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.226
-
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Homo sapiens
Application (protein specific)
Application
Commentary
Organism
medicine
BBOX is a therapeutic target in humans, the BBOX inhibitor mildronate is used to decrease fatty acid oxidation in patients after myocardial infarction
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene BBOX1, recombinant expression of His-tagged codon-optimized enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
recombinant expression of N-terminally His-tagged codon-optimized enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure analysis, PDB ID 3O2G
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(3R)-3-fluoro-4-(trimethylammonio)butanoate
-
Homo sapiens
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium
-
Homo sapiens
4-Trimethylammoniobutanoate
substrate inhibition
Homo sapiens
4-Trimethylammoniobutanoate
substrate inhibition at high concentrations
Pseudomonas sp.
mildronate
-
Homo sapiens
N-oxalylglycine
-
Homo sapiens
N-oxalylglycine
binding structure modeling
Pseudomonas sp.
Ni2+
binding structure modeling
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.024
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.125
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.136
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.226
-
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
additional information
-
additional information
Michaelis-Menten kinetics
Pseudomonas sp.
0.0042
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.02
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
0.02
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.153
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.163
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication, only the Michaelis-Menten range is fitted
Pseudomonas sp.
0.412
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.532
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.623
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
2.474
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication, the whole range is fitted
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
nonheme Fe2+-dependent oxygenase, dependent on, required for catalysis
Homo sapiens
Fe2+
nonheme Fe2+-dependent oxygenase, dependent on, required for catalysis
Pseudomonas sp.
additional information
Ni2+ cannot substitute for Fe2+
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Homo sapiens
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Pseudomonas sp.
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
Pseudomonas sp. AK-1
-
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography
Homo sapiens
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography, to over 90% purity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + formylfluoride
-
-
-
-
(R)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
N-[(3R)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(R)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
N-[(3R)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
N-[(3S)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-2-amino-3-carboxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
N-[(3S)-3-amino-3-carboxy-2-hydroxypropyl]-N,N-dimethylmethanaminium + succinate + CO2
-
-
-
?
(S)-3-carboxy-2-hydroxy-N,N,N-trimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
(3S)-3-carboxy-2,3-dihydroxy-N,N,N-trimethylpropan-1-aminium + succinate + CO2
-
-
-
?
2-carboxy-N,N,N-trimethylethan-1-aminium + 2-oxoglutarate + O2
-
745953
Homo sapiens
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
2-carboxy-N,N,N-trimethylethan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Homo sapiens
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium + succinate + CO2
-
-
-
?
3-carboxy-N-(fluoromethyl)-N,N-dimethylpropan-1-aminium + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp. AK-1
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
4-carboxy-3-hydroxy-N,N,N-trimethylbutan-1-aminium + succinate + CO2
-
-
-
?
4-trimethylammoniopropanoate + 2-oxoglutarate + O2
-
745953
Homo sapiens
2-carboxy-2-hydroxy-N,N,N-trimethylethan-1-aminium + succinate + CO2
-
-
-
?
D-carnitine + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
D-carnitine + 2-oxoglutarate + O2
-
745953
Pseudomonas sp.
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
L-carnitine + 2-oxoglutarate + O2
-
745953
Homo sapiens
3-carboxy-N,N,N-trimethyl-2-oxopropan-1-aminium + succinate + CO2
-
-
-
?
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Homo sapiens
?
-
-
-
-
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Pseudomonas sp.
?
-
-
-
-
additional information
no activity with trimethyllysine, acetylcholine, phosphocholine, thioacetylcholine, carbachol, and gamma-butyrobetaine analogues carboxy-N,N,N-trimethylmethanaminium, 5-carboxy-N,N,N-trimethylpentan-1-aminium, N,N,N-trimethyl-4-oxopentan-1-aminium, and 4-methoxy-N,N,N-trimethyl-4-oxobutan-1-aminium, substrate specificity, overview
745953
Pseudomonas sp. AK-1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
homology structure modelling
Homo sapiens
More
homology structure modelling
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.059
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
0.14
-
(3R)-3-fluoro-4-(trimethylammonio)butanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
0.3
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Homo sapiens
0.83
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Homo sapiens
1.6
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Homo sapiens
3.5
-
2-oxoglutarate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
5
-
(2S)-3-carboxy-N-(fluoromethyl)-2-hydroxy-N,N-dimethylpropan-1-aminium
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
21.3
-
4-Trimethylammoniobutanoate
pH 7.5, temperature not specified in the publication
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
7.5
-
assay at
Pseudomonas sp.
General Information
General Information
Commentary
Organism
evolution
comparison of properties, substrate specificities, and structures of the human enzyme (PDB ID 3O2G) and the enzyme from Pseudomonas sp. AK1, detailed overview
Homo sapiens
evolution
comparison of properties, substrate specificities, and structures of the human enzyme (PDB ID 3O2G) and the enzyme from Pseudomonas sp. AK1, detailed overview
Pseudomonas sp.
metabolism
gamma-butyrobetaine hydroxylase (BBOX) catalyses the final step in the biosynthesis of carnitine i.e. the stereoselective hydroxylation of gamma-butyrobetaine (GBB)
Homo sapiens
metabolism
gamma-butyrobetaine hydroxylase (BBOX) catalyses the final step in the biosynthesis of carnitine i.e. the stereoselective hydroxylation of gamma-butyrobetaine (GBB)
Pseudomonas sp.
additional information
homology structure modeling with bound Ni2+ and N-oxalglycine, modeled based on human BBOX structure, DB ID 2O2G
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
evolution
comparison of properties, substrate specificities, and structures of the human enzyme (PDB ID 3O2G) and the enzyme from Pseudomonas sp. AK1, detailed overview
Homo sapiens
evolution
comparison of properties, substrate specificities, and structures of the human enzyme (PDB ID 3O2G) and the enzyme from Pseudomonas sp. AK1, detailed overview
Pseudomonas sp.
metabolism
gamma-butyrobetaine hydroxylase (BBOX) catalyses the final step in the biosynthesis of carnitine i.e. the stereoselective hydroxylation of gamma-butyrobetaine (GBB)
Homo sapiens
metabolism
gamma-butyrobetaine hydroxylase (BBOX) catalyses the final step in the biosynthesis of carnitine i.e. the stereoselective hydroxylation of gamma-butyrobetaine (GBB)
Pseudomonas sp.
additional information
homology structure modeling with bound Ni2+ and N-oxalglycine, modeled based on human BBOX structure, DB ID 2O2G
Pseudomonas sp.
Other publictions for EC 1.14.11.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744717
Kamps
Cation-Pi interactions contri ...
Pseudomonas sp., Pseudomonas sp. AK-1
Chemistry
22
1270-1276
2016
1
-
1
-
-
-
9
4
-
1
-
2
-
3
-
-
1
-
-
-
-
-
13
-
1
-
-
3
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
9
-
4
-
1
-
2
-
-
-
1
-
-
-
-
13
-
1
-
-
3
1
-
-
-
-
2
2
-
-
-
745684
Khan
-
Development and application o ...
Homo sapiens
MedChemComm
7
873-880
2016
1
1
-
-
-
-
3
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
1
1
-
-
-
-
-
2
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
744503
Rydzik
Ejection of structural zinc l ...
Homo sapiens
Bioorg. Med. Chem. Lett.
24
4954-4957
2014
-
1
-
-
-
-
9
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
8
-
1
-
-
-
-
-
8
9
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
744690
Rydzik
Modulating carnitine levels b ...
Homo sapiens
Chem. Sci.
5
1765-1771
2014
-
-
-
1
-
-
16
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
1
-
-
13
16
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
745521
Tars
Targeting carnitine biosynthe ...
Homo sapiens
J. Med. Chem.
57
2213-2236
2014
1
-
1
1
-
-
54
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
51
1
-
1
-
1
-
-
51
54
1
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
745953
Rydzik
Comparison of the substrate s ...
Homo sapiens, Pseudomonas sp., Pseudomonas sp. AK-1
Org. Biomol. Chem.
12
6354-6358
2014
1
1
2
1
-
-
8
11
-
3
-
3
-
4
-
-
2
-
-
-
-
-
24
2
-
-
-
8
2
-
-
-
4
-
-
1
1
2
-
1
-
-
-
8
4
11
-
3
-
3
-
-
-
2
-
-
-
-
24
2
-
-
-
8
2
-
-
-
-
5
5
-
-
-
727308
Rydzik
Development and application of ...
Homo sapiens
ChemBioChem
13
1559-1563
2012
-
1
1
-
-
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
6
1
-
-
-
-
-
2
-
1
1
-
-
-
-
2
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
711108
Tars
Crystal structure of human gam ...
Homo sapiens
Biochem. Biophys. Res. Commun.
398
634-639
2010
-
-
1
1
-
-
2
1
-
2
-
1
-
2
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
1
2
-
1
-
2
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
695482
Fujita
Hepatic uptake of gamma-butyro ...
Rattus norvegicus
Am. J. Physiol. Gastrointest. Liver Physiol.
297
G681-G686
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697453
Fischer
Activities of gamma-butyrobeta ...
Sus scrofa
Comp. Biochem. Physiol. A
153
324-331
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
14
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
3
-
1
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
699163
Hirche
Determination of carnitine, it ...
Sus scrofa
J. Chromatogr. B
877
2158-2162
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699966
Garcia-Delgado
Ontogeny of Na(+)/L-carnitine ...
Rattus norvegicus
Mech. Ageing Dev.
130
227-233
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
5
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
686566
Ringseis
Clofibrate treatment up-regula ...
Sus scrofa
Eur. J. Pharmacol.
583
11-17
2008
-
1
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685321
van Vlies
PPARalpha-activation results i ...
Mus musculus
Biochim. Biophys. Acta
1767
1134-1142
2007
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671302
van Vlies
Measurement of carnitine biosy ...
Mus musculus
Anal. Biochem.
354
132-139
2006
-
-
-
-
-
-
1
2
-
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672375
Rigault
Genomic structure, alternative ...
Homo sapiens
Biochim. Biophys. Acta
1761
1469-1481
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675561
Davis
Carnitine deficiency and suppl ...
Rattus norvegicus
J. Nutr.
135
761-764
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675785
Debreceni
Effect of aromatic ring-contai ...
Rattus norvegicus
Metab. Clin. Exp.
54
1582-1586
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676337
Buermans
Microarray analysis reveals pi ...
Rattus norvegicus
Physiol. Genomics
21
314-323
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439302
Galland
Thyroid hormone controls carni ...
Rattus norvegicus
Cell. Mol. Life Sci.
59
540-545
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439300
Vaz
Carnitine biosynthesis. Purifi ...
Rattus norvegicus
Adv. Exp. Med. Biol.
466
117-124
1999
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439303
Galland
Molecular cloning and characte ...
Rattus norvegicus
Biochim. Biophys. Acta
1441
85-92
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
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