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Literature summary for 1.14.11.1 extracted from

  • Rydzik, A.M.; Leung, I.K.; Kochan, G.T.; Thalhammer, A.; Oppermann, U.; Claridge, T.D.; Schofield, C.J.
    Development and application of a fluoride-detection-based fluorescence assay for gamma-butyrobetaine hydroxylase (2012), ChemBioChem, 13, 1559-1563.
    View publication on PubMed
Search for more literature for 1.14.11.1

Application

Application Comment Organism
molecular biology a fluorescence assay based on the detection of fluoride released from reactions of fluorinated substrates with BBOX by the use of tert-butyldimethylsilyl-protected fluorescein is developed. (3S)-3-fluoro-4-(trimethylammonio)butanoate is a good substrate for BBOX, releasing fluoride when subjected to the enzyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein is used Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine
-
 Homo sapiens
Pyridine 2,4-dicarboxylate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 (3S)-3-fluoro-4-(trimethylammonio)butanoate co-substrate: 2-oxoglutarate, pH 7.5, 25°C, NMR assay Homo sapiens
0.026
-
 2-oxoglutarate co-substrate: 4-(trimethylammonio)butanoic acid, pH 7.5, 25°C, NMR assay Homo sapiens
0.083
-
 (3S)-3-fluoro-4-(trimethylammonio)butanoate co-substrate: 2-oxoglutarate, pH 7.5, 25°C, fluoride assay Homo sapiens
0.15
-
 2-oxoglutarate co-substrate: (3S)-3-fluoro-4-(trimethylammonio)butanoate, pH 7.5, 25°C, NMR assay Homo sapiens
0.16
-
 4-(trimethylammonio)butanoic acid co-substrate: 2-oxoglutarate, pH 7.5, 25°C, NMR assay Homo sapiens
0.47
-
 2-oxoglutarate co-substrate: (3S)-3-fluoro-4-(trimethylammonio)butanoate, pH 7.5, 25°C, fluoride assay Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2 weak substrate, below 5% turnover after 3 h Homo sapiens ?
-
 ?
(3S)-3-fluoro-4-(trimethylammonio)butanoate + 2-oxoglutarate + O2 S-stereoisomer preferred substrate Homo sapiens ?
-
 ?
4-(trimethylammonio)butanoic acid + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
gamma-butyrobetaine + 2-oxoglutarate + O2
-
 Homo sapiens carnitine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
BBOX
-
 Homo sapiens
gamma-butyrobetaine hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 (3S)-3-fluoro-4-(trimethylammonio)butanoate co-substrate: 2-oxoglutarate, pH 7.5, 25°C, NMR assay Homo sapiens
0.39
-
 2-oxoglutarate co-substrate: (3S)-3-fluoro-4-(trimethylammonio)butanoate, pH 7.5, 25°C, NMR assay Homo sapiens
0.55
-
 2-oxoglutarate co-substrate: (3S)-3-fluoro-4-(trimethylammonio)butanoate, pH 7.5, 25°C, fluoride assay Homo sapiens
0.62
-
 (3S)-3-fluoro-4-(trimethylammonio)butanoate co-substrate: 2-oxoglutarate, pH 7.5, 25°C, fluoride assay Homo sapiens
5.1
-
 2-oxoglutarate co-substrate: 4-(trimethylammonio)butanoic acid, pH 7.5, 25°C, NMR assay Homo sapiens
5.3
-
 4-(trimethylammonio)butanoic acid co-substrate: 2-oxoglutarate, pH 7.5, 25°C, NMR assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.018
-
 pH 7.5, 25°C Homo sapiens N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine
0.082
-
 pH 7.5, 25°C Homo sapiens Pyridine 2,4-dicarboxylate