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Literature summary for 1.13.99.1 extracted from

  • Zheng, S.; Hou, J.; Zhou, Y.; Fang, H.; Wang, T.T.; Liu, F.; Wang, F.S.; Sheng, J.Z.
    One-pot two-strain system based on glucaric acid biosensor for rapid screening of myo-inositol oxygenase mutations and glucaric acid production in recombinant cells (2018), Metab. Eng., 49, 212-219 .
    View publication on PubMed
Search for more literature for 1.13.99.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain EA31 and Saccharomyces cerevisiae strain SG Mus musculus

Protein Variants

Protein Variants Comment Organism
D82Y the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme Mus musculus
L240M the mutant's activity shows a drastic improvement compared with the wild type enzyme Mus musculus
Q62H the mutant displays significantly decreased Km values and lower kcat value compared to the wild type enzyme Mus musculus
Q62H/S173N/L240M the mutant's activity shows a drastic improvement compared with the wild type enzyme Mus musculus
S173N the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.105
-
 myo-inositol mutant enzyme S173N, at 37°C, pH not specified in the publication Mus musculus
2.516
-
 myo-inositol mutant enzyme D82Y/S173N/L240M, at 37°C, pH not specified in the publication Mus musculus
2.905
-
 myo-inositol mutant enzyme Q62H, at 37°C, pH not specified in the publication Mus musculus
3.573
-
 myo-inositol mutant enzyme D82Y, at 37°C, pH not specified in the publication Mus musculus
4.55
-
 myo-inositol wild type enzyme, at 37°C, pH not specified in the publication Mus musculus
7.629
-
 myo-inositol mutant enzyme L240M, at 37°C, pH not specified in the publication Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + O2 Mus musculus
-
 D-glucuronate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9QXN5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + O2
-
 Mus musculus D-glucuronate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MIOX
-
 Mus musculus
Myo-inositol oxygenase
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
59.5
-
 myo-inositol mutant enzyme Q62H, at 37°C, pH not specified in the publication Mus musculus
76.8
-
 myo-inositol wild type enzyme, at 37°C, pH not specified in the publication Mus musculus
85.1
-
 myo-inositol mutant enzyme S173N, at 37°C, pH not specified in the publication Mus musculus
109.9
-
 myo-inositol mutant enzyme L240M, at 37°C, pH not specified in the publication Mus musculus
110.8
-
 myo-inositol mutant enzyme D82Y/S173N/L240M, at 37°C, pH not specified in the publication Mus musculus
129.4
-
 myo-inositol mutant enzyme D82Y, at 37°C, pH not specified in the publication Mus musculus