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Literature summary for 1.13.99.1 extracted from

  • Teng, F.; You, R.; Hu, M.; Liu, W.; Wang, L.; Tao, Y.
    Production of D-glucuronic acid from myo-inositol using Escherichia coli whole-cell biocatalyst overexpressing a novel myo-inositol oxygenase from Thermothelomyces thermophile (2019), Enzyme Microb. Technol., 127, 70-74 .
    View publication on PubMed
Search for more literature for 1.13.99.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BW25113 Mus musculus
expressed in Escherichia coli strain BW25113 Thermothelomyces thermophilus
expressed in Escherichia coli strain BW25113 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 2 mM used in assay conditions Mus musculus
Fe2+ 2 mM used in assay conditions Thermothelomyces thermophilus
Fe2+ 2 mM used in assay conditions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + O2 Mus musculus
-
 D-glucuronate + H2O
-
 ?
myo-inositol + O2 Arabidopsis thaliana
-
 D-glucuronate + H2O
-
 ?
myo-inositol + O2 Thermothelomyces thermophilus conversion rate of 91.3% D-glucuronate + H2O
-
 ?
myo-inositol + O2 Thermothelomyces thermophilus ATCC 42464 conversion rate of 91.3% D-glucuronate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana A0A178V136
-
-
Mus musculus Q9QXN5
-
-
Thermothelomyces thermophilus G2QA66
-
-
Thermothelomyces thermophilus ATCC 42464 G2QA66
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mus musculus
Ni-NTA column chromatography Thermothelomyces thermophilus
Ni-NTA column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
 at pH 7.0 and 37°C Arabidopsis thaliana
5
-
 at pH 7.0 and 37°C Mus musculus
5.1
-
 at pH 7.0 and 37°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + O2
-
 Mus musculus D-glucuronate + H2O
-
 ?
myo-inositol + O2
-
 Arabidopsis thaliana D-glucuronate + H2O
-
 ?
myo-inositol + O2 conversion rate of 91.3% Thermothelomyces thermophilus D-glucuronate + H2O
-
 ?
myo-inositol + O2 conversion rate of 91.3% Thermothelomyces thermophilus ATCC 42464 D-glucuronate + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * about 40000, SDS-PAGE Mus musculus
? x * about 40000, SDS-PAGE Arabidopsis thaliana
? x * about 38000, SDS-PAGE Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
MIOX
-
 Mus musculus
MIOX
-
 Thermothelomyces thermophilus
MIOX
-
 Arabidopsis thaliana
Myo-inositol oxygenase
-
 Mus musculus
Myo-inositol oxygenase
-
 Thermothelomyces thermophilus
Myo-inositol oxygenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Arabidopsis thaliana
37
-
-
 Mus musculus
37
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 enzyme maintains approximately 75% of its highest activity at a broad range from 20°C to 37°C. The activity decreases rapidly when the temperature is higher than 50°C Mus musculus
20 37 enzyme maintains approximately 75% of its highest activity at a broad range from 20°C to 37°C. The activity decreases rapidly when the temperature is higher than 50°C Thermothelomyces thermophilus
20 37 enzyme maintains approximately 75% of its highest activity at a broad range from 20°C to 37°C. The activity decreases rapidly when the temperature is higher than 50°C Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the enzyme exhibits approximately 70% of its activity after incubation at 37°C for 40 min and possesses 30% of its initial activity after incubation at 37°C for 1 h Thermothelomyces thermophilus
37
-
 the enzyme shows less than 40% of its activity at 37 °C for 40 min Mus musculus
37
-
 the enzyme shows less than 40% of its activity at 37 °C for 40 min Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arabidopsis thaliana
8
-
-
 Mus musculus
8
-
-
 Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 the enzyme keeps more than 70% of the highest activity at a range from pH 7.0-8.5. When the pH is lower than 6.0, the activity decreases rapidly Arabidopsis thaliana
7 8.5 the enzyme shows high activity (more than 70%) across the pH range from 7.5-8.5 Mus musculus
7.5 8.5 the enzyme shows high activity (more than 70%) across the pH range from 7.5-8.5 Thermothelomyces thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 the enzyme retains more than 70% activity after 30 min at pH 6.0-8.0. After 30 min at pH 9.0, the enzyme activity drops to 50% of the original value Mus musculus
6 8 the enzyme retains more than 70% activity after 30 min at pH 6.0-8.0. After 30 min at pH 9.0, the enzyme activity drops to 50% of the original value Thermothelomyces thermophilus
6 8 the enzyme retains more than 70% activity after 30 min at pH 6.0-8.0. After 30 min at pH 9.0, the enzyme activity drops to 50% of the original value Arabidopsis thaliana