Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.99.1 extracted from

  • Arner, R.J.; Prabhu, K.S.; Thompson, J.T.; Hildenbrandt, G.R.; Liken, A.D.; Reddy, C.C.
    myo-Inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol (2001), Biochem. J., 360, 313-320.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cysteine 1 mM Fe2+ plus 2 mM cysteine required Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
open reading frame of 849 base pairs Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.9
-
myo-inositol with Fe(2+) and L-cysteine as activators Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM Fe2+ plus 2mM cysteine required Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32663
-
electrospray MS determination, gel filtration, SDS-PAGE, sedimentation equilibirum, enzyme does not undergo oligomerization in the presence of myo-inositol Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + O2 Sus scrofa major role in pathogenesis of diabetis d-glucuronate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa Q8WN98 SwissProt Accession Code:Q8WN98
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-chiro-inositol + O2 much less active with D-chiro-inositol than with myo-inositol Sus scrofa D-glucuronate
-
?
myo-inositol + O2 highly specific for myo-inositol Sus scrofa D-glucuronate + H2O
-
?
myo-inositol + O2 major role in pathogenesis of diabetis Sus scrofa d-glucuronate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32663, electrospray MS determination, gel filtration, SDS-PAGE, sedimentation equilibirum, enzyme does not undergo oligomerization in the presence of myo-inositol Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.183
-
myo-inositol with Fe(2+) and L-cysteine as activators Sus scrofa