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Literature summary for 1.13.12.9 extracted from

  • Koyama, H.
    Further characterization of a novel L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501 (1983), J. Biochem., 93, 1313-1319.
    View publication on PubMed
Search for more literature for 1.13.12.9

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline slight Pseudomonas sp.
2,2'-dipyridyl slight Pseudomonas sp.
8-hydroxyquinoline slight Pseudomonas sp.
CuSO4 preferentially inhibits oxidase activity versus oxygenase activity Pseudomonas sp.
FeSO4 preferentially inhibits oxidase activity versus oxygenase activity Pseudomonas sp.
HgCl2
-
 Pseudomonas sp.
ZnSO4
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0107
-
 L-Phe
-
 Pseudomonas sp.
1.82
-
 O2
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 2 * 68000, SDS-PAGE Pseudomonas sp.
140000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 P-501
-
Pseudomonas sp. P-501
-
 P-501
-

Storage Stability

Storage Stability Organism
-20°C, 1 mM or 10 mM potassium phosphate buffer, pH 7.0, stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe + O2
-
 Pseudomonas sp. 2-phenylacetamide + CO2 + H2O
-
 ?
L-Phe + O2
-
 Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 68000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 formation of alpha-phenylacetamide Pseudomonas sp.
65
-
 formation of beta-phenylpyruvate Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 65% of maximal activity, 70°C: about 50% of maximal activity, formation of alpha-phenylacetamide Pseudomonas sp.
40 85 40°C: about 50% of maximal activity 40°C and 85°C, formation of beta-phenylpyruvate Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, no loss of activity Pseudomonas sp.
80
-
 10 min, 46% loss of activity Pseudomonas sp.
85
-
 10 min, 90% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9.5 formation of alpha-phenylacetamide Pseudomonas sp.
11
-
 formation of beta-phenylpyruvate Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
9.8 11.8 50% of maximal activity at pH 9.8 and 11.8, formation of beta-phenylpyruvate Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD contains 2 mol of FAD per mol of enzyme Pseudomonas sp.