Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lampyris turkestanicus |
Protein Variants | Comment | Organism |
---|---|---|
L300E | specific activity is increased and Km-value is increased about 1.7fold compared with the wild-type enzyme | Lampyris turkestanicus |
L300K | relative stability of mutant luciferase increases compared with native luciferase, mutation causes no effects on bioluminescence spectrum | Lampyris turkestanicus |
L300R | relative stability of mutant luciferase increases compared with native luciferase, mutation causes no effects on bioluminescence spectrum, specific activity is increased as compared to wild-type enzyme | Lampyris turkestanicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
D-firefly luciferin | pH and temperature not specified in the publication, mutant enzyme L300K | Lampyris turkestanicus | |
12.5 | - |
D-firefly luciferin | pH and temperature not specified in the publication, wild-type enzyme | Lampyris turkestanicus | |
14 | - |
D-firefly luciferin | pH and temperature not specified in the publication, mutant enzyme L300R | Lampyris turkestanicus | |
25 | - |
D-firefly luciferin | pH and temperature not specified in the publication, mutant enzyme L300E | Lampyris turkestanicus | |
83 | - |
ATP | pH and temperature not specified in the publication, wild-type enzyme | Lampyris turkestanicus | |
88 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme L300K | Lampyris turkestanicus | |
100 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme L300R | Lampyris turkestanicus | |
140 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme L300E | Lampyris turkestanicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-firefly luciferin + O2 + ATP | Lampyris turkestanicus | - |
firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lampyris turkestanicus | Q5UFR2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Lampyris turkestanicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-firefly luciferin + O2 + ATP | - |
Lampyris turkestanicus | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
wild-type enzyme, mutant enzyme L300K and mutant enzyme L300E | Lampyris turkestanicus |
30 | - |
mutant enzyme L300R | Lampyris turkestanicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
wild-type enzyme, mutant enzyme L300K, mutant enzyme L300E and mutant enzyme L300R | Lampyris turkestanicus |