Protein Variants | Comment | Organism |
---|---|---|
K329I | point mutation does not affect the orientation of critical residues in bioluminescence color determination. Thermostability and Km value for luciferin are decreased as compared to wild type enzyme. Intrinsic fluorescence and far-UV CD intensity in K329I mutant is decreased. Realative activity as compared to wild-type enzyme is 32% | Photinus pyralis |
R330Q | point mutation does not affect the orientation of critical residues in bioluminescence color determination. Thermostability and Km value for luciferin are decreased as compared to wild type enzyme. Increase in tryptophan fluorescence intensity and secondary structure content for R330Q as compared with wild type. Realative activity as compared to wild-type enzyme is 23% | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-firefly luciferin | pH 7.8, 25°C, wild-type enzyme | Photinus pyralis | |
0.0225 | - |
D-firefly luciferin | pH 7.8, 25°C, mutant enzyme K329I | Photinus pyralis | |
0.05 | - |
ATP | pH 7.8, 25°C, wild-type enzyme | Photinus pyralis | |
0.05 | - |
ATP | pH 7.8, 25°C, mutant enzyme R330Q | Photinus pyralis | |
0.075 | - |
ATP | pH 7.8, 25°C, mutant enzyme K329I | Photinus pyralis | |
0.1421 | - |
D-firefly luciferin | pH 7.8, 25°C, mutant enzyme R330Q | Photinus pyralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-firefly luciferin + O2 + ATP | Photinus pyralis | - |
firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | P08659 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-firefly luciferin + O2 + ATP | - |
Photinus pyralis | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
wild-type enzyme and mutant enzymes R330Q and K329I | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | pH 7.8, 25°C, mutant enzyme K329I | Photinus pyralis |
7.5 | 8 | pH 7.8, 25°C, wild-type enzyme | Photinus pyralis |
8 | - |
pH 7.8, 25°C, mutant enzyme R330Q | Photinus pyralis |