Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
A296C/A326C | specific activity 676% (compared to wild-type 100%), Km (ATP) decreased compared to wild-type, Km (D-luciferin) decreased compared to wild-type, optimal temperature 35° (wild-type 25°C), optimal pH 8.5 (wild-type pH 8), activity remains 54% at 40°C for 5 min (compared to wild-type 0%), t1/2: 40 min at 35°C (compared to wild-type 5 min) | Photinus pyralis |
I232R | specific activity 75% (compared to wild-type 100%), Km (ATP) increased compared to wild-type, Km (D-luciferin) increased compared to wild-type, optimal temperature 25° (wild-type 25°C), optimal pH 8.5 (wild-type pH 8), activity remains 4% at 40°C for 5 min (compared to wild-type 0%), t1/2: 20 min at 35°C (compared to wild-type 5 min) | Photinus pyralis |
I232R/A296C/A326C | specific activity 358% (compared to wild-type 100%), Km (ATP) increased compared to wild-type, Km (D-luciferin) increased compared to wild-type, optimal temperature 35° (wild-type 25°C), optimal pH 8 (wild-type pH 8), activity remains 28% at 40°C for 5 min (compared to wild-type 0%), t1/2: 10 min at 35°C (compared to wild-type 5 min) | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ATP | 35°C, pH 8.5, mutant A296C/A326C | Photinus pyralis | |
0.1 | - |
D-luciferin | 35°C, pH 8.5, mutant A296C/A326C | Photinus pyralis | |
0.125 | - |
ATP | 25°C, pH 8, wild-type | Photinus pyralis | |
0.125 | - |
D-luciferin | 25°C, pH 8, wild-type | Photinus pyralis | |
0.2 | - |
ATP | 25°C, pH 8.5, mutant I232R | Photinus pyralis | |
0.2 | - |
D-luciferin | 25°C, pH 8.5, mutant I232R | Photinus pyralis | |
0.2 | - |
ATP | 35°C, pH 8, mutant A296C/A326C/I232R | Photinus pyralis | |
0.2 | - |
D-luciferin | 35°C, pH 8, mutant A296C/A326C/I232R | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
luciferin | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
T1/2 results show that half-lives (t1/2) of wild-type, mutant A296C/A326C, mutant A296C/A326C/I232R and mutant I232R are 5, 40, 10 and 20 min at 35°C respectively | Photinus pyralis |
40 | - |
thermal stability experiments of mutant A296C/A326C, mutant A296C/A326C/I232R and mutant I232R shows that their original activity remains approximately 54%, 28%, and 4% after incubation at 40°C for 5 min, respectively (compared to wild-type 0%) | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Photinus pyralis |