Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 and XL1blue | Luciola mingrelica |
Protein Variants | Comment | Organism |
---|---|---|
F16L | lower pH-dependence of bioluminescence spectra, about 40% decrease in specific activity | Luciola mingrelica |
F16L/A40S | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 80% decrease in specific activity | Luciola mingrelica |
S118C | increased thermostability, about 30% increase in specific activity | Luciola mingrelica |
Y35H | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 40% decrease in specific activity | Luciola mingrelica |
Y35N | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 30% decrease in specific activity | Luciola mingrelica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Luciola mingrelica | Q26304 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Luciola mingrelica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luciferin + ATP + O2 | - |
Luciola mingrelica | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Luciola mingrelica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
shift in bioluminescence spectra to red region by increase in temperature to 42°C | Luciola mingrelica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
50% decrease in activity after 6.9 min for wild-type luciferase, for mutant S118C after 13.4 min | Luciola mingrelica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at, shift in bioluminescence spectra to red region by increase in temperature to 42°C | Luciola mingrelica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.8 | bioluminescence spectra independent from pH, for mutants Y35N, Y35H and F16L/A40S | Luciola mingrelica |