Cloned (Comment) | Organism |
---|---|
mutant enzymes,R218A, H245A, G246A, F247A, F247L, F247Y, F250G, F250S, T251A, G315A, G316A, G341A, L342A, T343A, S347A, A348V, I351A and K529A | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
A348V | Km-value for D-luciferin is 8.9fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 2fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 6.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
F247A | Km-value for D-luciferin is 15.3fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 17.9fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 21.9fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
F247L | Km-value for D-luciferin is 8.3fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is nearly identical to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.4fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
F247Y | Km-value for D-luciferin is 1.2fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 128fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 2fold lower compared to the Km-value of the wild-type enzyme | Photinus pyralis |
F250G | Km-value for D-luciferin is neraly identical to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 9.6fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is nearly identical to the Km-value of the wild-type enzyme | Photinus pyralis |
F250S | Km-value for D-luciferin is 1.5fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 3.5fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.2fold lower compared to the Km-value of the wild-type enzyme | Photinus pyralis |
G246A | Km-value for D-luciferin is 3.6fold lower compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 2.1fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 5.5fold lower compared to the Km-value of the wild-type enzyme | Photinus pyralis |
G315A | Km-value for D-luciferin is 13.3fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 625fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 7.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
G316A | Km-value for D-luciferin is 1.2fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 2.6fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.5fold lower compared to the Km-value of the wild-type enzyme | Photinus pyralis |
G341A | Km-value for D-luciferin is 4fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 625fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.3fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
H245A | Km-value for D-luciferin is identical to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 3fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
I351A | Km-value for D-luciferin is 5.7fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 1.5fold higher compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 1.8fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
K529A | Km-value for D-luciferin is 15.3fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 1250fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 7.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
L342A | Km-value for D-luciferin is 8.7fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 1.2fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 7.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
R218A | Km-value for D-luciferin is 20fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 31.3fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 38.8fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
S347A | Km-value for D-luciferin is 11.3fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 4.2fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 2.2fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
T251A | Km-value for D-luciferin is 20.7fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 2.9fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 12.5fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
T343A | Km-value for D-luciferin is 6.6fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 125fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 5.4fold higher compared to the Km-value of the wild-type enzyme | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
D-luciferin | mutant enzyme G246A | Photinus pyralis | |
0.015 | - |
D-luciferin | wild-type enzyme | Photinus pyralis | |
0.015 | - |
D-luciferin | mutant enzyme H245A | Photinus pyralis | |
0.0157 | - |
D-luciferin | mutant enzyme F250G | Photinus pyralis | |
0.018 | - |
D-luciferin | mutant enzyme F247Y | Photinus pyralis | |
0.018 | - |
D-luciferin | mutant enzyme G316A | Photinus pyralis | |
0.022 | - |
D-luciferin | mutant enzyme F250S | Photinus pyralis | |
0.029 | - |
MgATP2- | mutant enzyme G246A | Photinus pyralis | |
0.06 | - |
D-luciferin | mutant enzyme G341A | Photinus pyralis | |
0.08 | - |
MgATP2- | mutant enzyme F247Y | Photinus pyralis | |
0.085 | - |
D-luciferin | mutant enzyme I351A | Photinus pyralis | |
0.099 | - |
D-luciferin | mutant enzyme T343A | Photinus pyralis | |
0.107 | - |
MgATP2- | mutant enzyme G316A | Photinus pyralis | |
0.124 | - |
D-luciferin | mutant enzyme F247L | Photinus pyralis | |
0.13 | - |
D-luciferin | mutant enzyme L342A | Photinus pyralis | |
0.133 | - |
D-luciferin | mutant enzyme A348V | Photinus pyralis | |
0.133 | - |
MgATP2- | mutant enzyme F250S | Photinus pyralis | |
0.16 | - |
MgATP2- | wild-type enzyme | Photinus pyralis | |
0.166 | - |
MgATP2- | mutant enzyme F250G | Photinus pyralis | |
0.17 | - |
D-luciferin | mutant enzyme S347A | Photinus pyralis | |
0.2 | - |
D-luciferin | mutant enzyme G315A | Photinus pyralis | |
0.2 | - |
MgATP2- | mutant enzyme G341A | Photinus pyralis | |
0.226 | - |
MgATP2- | mutant enzyme F247L | Photinus pyralis | |
0.23 | - |
D-luciferin | mutant enzyme F247A | Photinus pyralis | |
0.23 | - |
D-luciferin | mutant enzyme K529A | Photinus pyralis | |
0.24 | - |
MgATP2- | mutant enzyme H245A | Photinus pyralis | |
0.285 | - |
MgATP2- | mutant enzyme I351A | Photinus pyralis | |
0.301 | - |
D-luciferin | mutant enzyme R218A | Photinus pyralis | |
0.311 | - |
D-luciferin | mutant enzyme T251A | Photinus pyralis | |
0.344 | - |
MgATP2- | mutant enzyme S347A | Photinus pyralis | |
0.857 | - |
MgATP2- | mutant enzyme T343A | Photinus pyralis | |
1.038 | - |
MgATP2- | mutant enzyme A348V | Photinus pyralis | |
1.2 | - |
MgATP2- | mutant enzyme G315A | Photinus pyralis | |
1.2 | - |
MgATP2- | mutant enzyme K529A | Photinus pyralis | |
1.2 | - |
MgATP2- | mutant enzyme L342A | Photinus pyralis | |
2 | - |
MgATP2- | mutant enzyme T251A | Photinus pyralis | |
3.5 | - |
MgATP2- | mutant enzyme F247A | Photinus pyralis | |
6.2 | - |
MgATP2- | mutant enzyme R218A | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
luciferin + MgATP2- | - |
Photinus pyralis | AMP + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
D-luciferin | mutant enzyme K529A | Photinus pyralis | |
0.0002 | - |
D-luciferin | mutant enzyme G315A | Photinus pyralis | |
0.0002 | - |
D-luciferin | mutant enzyme G341A | Photinus pyralis | |
0.001 | - |
D-luciferin | mutant enzyme T343A | Photinus pyralis | |
0.004 | - |
D-luciferin | mutant enzyme R218A | Photinus pyralis | |
0.007 | - |
D-luciferin | mutant enzyme F247A | Photinus pyralis | |
0.013 | - |
D-luciferin | mutant enzyme F250G | Photinus pyralis | |
0.03 | - |
D-luciferin | mutant enzyme S347A | Photinus pyralis | |
0.036 | - |
D-luciferin | mutant enzyme F250S | Photinus pyralis | |
0.041 | - |
D-luciferin | mutant enzyme H245A | Photinus pyralis | |
0.043 | - |
D-luciferin | mutant enzyme T251A | Photinus pyralis | |
0.049 | - |
D-luciferin | mutant enzyme G316A | Photinus pyralis | |
0.06 | - |
D-luciferin | mutant enzyme G246A | Photinus pyralis | |
0.064 | - |
D-luciferin | mutant enzyme A348V | Photinus pyralis | |
0.098 | - |
D-luciferin | mutant enzyme F247Y | Photinus pyralis | |
0.106 | - |
D-luciferin | mutant enzyme L342A | Photinus pyralis | |
0.116 | - |
D-luciferin | mutant enzyme F247L | Photinus pyralis | |
0.125 | - |
D-luciferin | wild-type enzyme | Photinus pyralis | |
0.19 | - |
D-luciferin | mutant enzyme I351A | Photinus pyralis |