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Literature summary for 1.13.12.7 extracted from

  • Leach F.R.
    ATP determination with firefly luciferase (1981), J. Appl. Biochem., 3, 473-517.
No PubMed abstract available

General Stability

General Stability Organism
bovine serum albumin stabilizes Photinus pyralis
EDTA stabilizes Photinus pyralis
EDTA, 2-mercaptoethanol, dithiothreitol and bovine serum albumin used as stabilizers Photinus pyralis
stabilized by immobilization on polysaccharide carriers Photinus pyralis
stabilized by immobilization on polysaccharide carriers Luciola mingrelica

Inhibitors

Inhibitors Comment Organism Structure
3',5'-cyclic AMP competitive inhibitor Photinus pyralis
5-anilino-1-naphthalene sulfonate competitive inhibitor Photinus pyralis
6-toluidino-2-naphthalene sulfonate competitive inhibitor Photinus pyralis
arsenate
-
Photinus pyralis
ATP
-
Photinus pyralis
dehydroluciferin competitive inhibitor Photinus pyralis
ethanol
-
Photinus pyralis
ethyl-2-benzothiazole sulfonate competitive inhibitor Photinus pyralis
L-1-tosylamido-2-phenethyl chlorometyl ketone competitive inhibitor with respect to luciferin, noncompetitive with respect to ATP Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
ATP crude extract Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000 52000 high speed equilibrium sedimentation after 5 M guanidine hydrochloride Photinus pyralis
89000 91000 low speed equilibrium sedimentation Photinus pyralis
92000 95000 high speed equilibrium sedimentation Photinus pyralis
100000
-
from sedimentation velocity of the crystalline form Photinus pyralis

Organism

Organism UniProt Comment Textmining
Luciola mingrelica
-
-
-
Photinus pyralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on DEAE-cellulose Luciola mingrelica
several chromatographic and non chromatographic methods Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
-
Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000-53000, various methods Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
purified Photinus pyralis
25
-
-
Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH 7.8 crude extract Photinus pyralis
7.8
-
purified enzyme Photinus pyralis

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
Photinus pyralis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
dehydroluciferin
-
Photinus pyralis
0.0059
-
6-toluidino-2-naphthalene sulfonate
-
Photinus pyralis
0.23
-
5-anilino-1-naphthalene sulfonate
-
Photinus pyralis
0.25
-
L-tosylamido-2-phenethyl chloromethyl ketone
-
Photinus pyralis