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Literature summary for 1.13.12.7 extracted from
- Purification and properties of firefly luciferase (1978), Methods Enzymol., 57, 3-15.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| extraction, 2 gel elutions, 5 crystallizations | Photinus pyralis |
General Stability
| General Stability | Organism |
|---|---|
| stability in crude solutions varies | Photinus pyralis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | lowers the flash height and extends the light emission for a given amount of ATP | Photinus pyralis |  |
| ATP | - |
Photinus pyralis | |
| diphosphate | - |
Photinus pyralis | |
| Luciferin | - |
Photinus pyralis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activator with optimum concentration at 0.1 mM | Photinus pyralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
- |
Photinus pyralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| several chromatographic and non chromatographic methods | Photinus pyralis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| whole body | - |
Photinus pyralis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
28000 light units/mg after crystallization | Photinus pyralis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.5, ammonium sulfate, EDTA, as 10 mg protein/ml slurry, several months | Photinus pyralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Photinus luciferin + O2 + ATP | - |
Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000 not separated | Photinus pyralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Photinus pyralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | pH up to 8 red light occurs instead of yellow-green light | Photinus pyralis |
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