Application | Comment | Organism |
---|---|---|
analysis | the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays | Cypridina noctiluca |
molecular biology | the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays | Cypridina noctiluca |
Cloned (Comment) | Organism |
---|---|
recombinant mutant enzyme lacking N-glycosylation motifs is produced using Expi293F cells, silkworms, and tobacco BY-2 cells. The relative activity of the produced Dmt CLucEX is almost the same as that of the wild-type CLucEX, whereas recombinant CLuc deficient in N-glycosylation binding sites (Dmt CLucCO) is approximately 20% of that of wild-type CLucCO. In the case of the silkworm system, the relative activity of mutant CLuc purified using only anti-FLAG affinity gel is 25% of that of wild-type CLucSW. However, further purification reveals that approximately 75% of the FLAG purified recombinant protein is aggregated and its enzyme activity is lost. In the BY-2 cells, attempt to express Dmt CLucBY result in no recombinant protein production despite the use of 4 independent transgenic callus clones. The productivity of CLuc mutated in the N-glycosylation sites differs depending on the expression host | Cypridina noctiluca |
Protein Variants | Comment | Organism |
---|---|---|
T184A/N404D | both N-glycosylation sites are mutated and lost | Cypridina noctiluca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cypridina luciferin + O2 | Cypridina noctiluca | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cypridina noctiluca | Q75R40 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation sites: T184 and N404 | Cypridina noctiluca |
Purification (Comment) | Organism |
---|---|
- |
Cypridina noctiluca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cypridina luciferin + O2 | - |
Cypridina noctiluca | oxidized Cypridina luciferin + CO2 + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CLuc | - |
Cypridina noctiluca |
Cypridina noctiluca luciferase | - |
Cypridina noctiluca |