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Literature summary for 1.13.12.6 extracted from
- Chemistry of the luciferases of Cypridina hilgendorfii and Apogon ellioti (2015), Biolumin. Progr., 2015, 137-149 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cypndina gamma-globulin | Cypridina luciferase is completely inhibited by a rabbit antibody to Cypridina luciferase | Vargula hilgendorfii | |
| additional information | Apogon luciferase is not inhibited by a rabbit antibody to Cypridina luciferase | Apogon ellioti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Vargula hilgendorfii | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Apogon ellioti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cypridina luciferin + O2 | Vargula hilgendorfii | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? |  |
| Cypridina luciferin + O2 | Apogon ellioti | - |
oxidized Cypridina luciferin + CO2 + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apogon ellioti | - |
- |
- |
| Vargula hilgendorfii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration | Vargula hilgendorfii |
| native enzyme from photogenic organs by fractional precipitation with acetone and ammonium sulfate, and dialysis, followed by gel filtration | Apogon ellioti |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| photogenic organ | - |
Vargula hilgendorfii | - |
| photogenic organ | - |
Apogon ellioti | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| Cypridina luciferase occasionally loses almost all of its activity when chromatographed through the 90-cm Sephadex column, irrespective of whether the column is newly poured or has been used for a period. The loss also occurs with both newly dissolved and old solutions of Cypridina luciferase | Vargula hilgendorfii |
| purified native Apogon luciferase gradually loses activity on standing in the cold over a period of weeks | Apogon ellioti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cypridina luciferin + O2 | - |
Vargula hilgendorfii | oxidized Cypridina luciferin + CO2 + hv | - |
? | |
| Cypridina luciferin + O2 | - |
Apogon ellioti | oxidized Cypridina luciferin + CO2 + hv | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Apogon luciferase | - |
Apogon ellioti |
| Apogon luciferase 1 | - |
Apogon ellioti |
| Cypridina luciferase | - |
Vargula hilgendorfii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0147 | - |
Cypridina luciferin | pH 6.8, 26°C | Vargula hilgendorfii | |
| 0.0152 | - |
Cypridina luciferin | pH 6.8, 26°C | Apogon ellioti | |
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