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Literature summary for 1.13.12.5 extracted from

  • Inouye, S.; Sahara-Miura, Y.; Sato, J.; Iimori, R.; Yoshida, S.; Hosoya, T.
    Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines (2013), Protein Expr. Purif., 88, 150-156.
    View publication on PubMed
Search for more literature for 1.13.12.5

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain (19 kDa) is expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Oplophorus gracilirostris
expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Renilla reniformis
expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Gaussia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 catalytic domain Oplophorus gracilirostris
37600
-
 SDS-PAGE Renilla reniformis
37700
-
 calculated from sequence Renilla reniformis
38040
-
 calculated from sequence, 4red-shift luciferase Renilla reniformis

Organism

Organism UniProt Comment Textmining
Gaussia
-
-
-
Oplophorus gracilirostris
-
-
-
Renilla reniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-chelate column Renilla reniformis
using Ni-chelate column Oplophorus gracilirostris
using Ni-chelate column Gaussia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3iso-coelenterazine + O2 relative activity to native coelenterazine: 11.6% Renilla reniformis ?
-
 ?
3iso-coelenterazine + O2 relative activity to native coelenterazine: 14.3% Gaussia ?
-
 ?
3iso-coelenterazine + O2 relative activity to native coelenterazine: 34.9% Renilla reniformis ?
-
 ?
3iso-coelenterazine + O2 relative activity to native coelenterazine: 78.2% Oplophorus gracilirostris ?
-
 ?
3me-coelenterazine + O2 relative activity to native coelenterazine: 11.8% Renilla reniformis ?
-
 ?
3me-coelenterazine + O2 relative activity to native coelenterazine: 2.6% Gaussia ?
-
 ?
3me-coelenterazine + O2 relative activity to native coelenterazine: 45.5% Renilla reniformis ?
-
 ?
3me-coelenterazine + O2 relative activity to native coelenterazine: 80% Oplophorus gracilirostris ?
-
 ?
3meo-coelenterazine + O2 relative activity to native coelenterazine: 189% Oplophorus gracilirostris ?
-
 ?
3meo-coelenterazine + O2 relative activity to native coelenterazine: 3.8% Gaussia ?
-
 ?
3meo-coelenterazine + O2 relative activity to native coelenterazine: 5.5% Renilla reniformis ?
-
 ?
3meo-coelenterazine + O2 relative activity to native coelenterazine: 82.3% Renilla reniformis ?
-
 ?
alphameh-coelenterazine + O2 relative activity to native coelenterazine: 0.02% Renilla reniformis ?
-
 ?
alphameh-coelenterazine + O2 relative activity to native coelenterazine: 0.02% Gaussia ?
-
 ?
alphameh-coelenterazine + O2 relative activity to native coelenterazine: 15.7% Oplophorus gracilirostris ?
-
 ?
alphameh-coelenterazine + O2 relative activity to native coelenterazine: 24.7% Renilla reniformis ?
-
 ?
cf3-coelenterazine + O2 relative activity to native coelenterazine: 0.8% Gaussia ?
-
 ?
cf3-coelenterazine + O2 relative activity to native coelenterazine: 16.8% Renilla reniformis ?
-
 ?
cf3-coelenterazine + O2 relative activity to native coelenterazine: 49.5% Oplophorus gracilirostris ?
-
 ?
cf3-coelenterazine + O2 relative activity to native coelenterazine: 5% Renilla reniformis ?
-
 ?
coelenterazine + O2 relative activity: 100% Renilla reniformis ?
-
 ?
coelenterazine + O2 relative activity: 100% Oplophorus gracilirostris ?
-
 ?
coelenterazine + O2 relative activity: 100% Gaussia ?
-
 ?
et-coelenterazine + O2 relative activity to native coelenterazine: 0.9% Gaussia ?
-
 ?
et-coelenterazine + O2 relative activity to native coelenterazine: 1.2% Renilla reniformis ?
-
 ?
et-coelenterazine + O2 relative activity to native coelenterazine: 21.1% Oplophorus gracilirostris ?
-
 ?
et-coelenterazine + O2 relative activity to native coelenterazine: 78.3% Renilla reniformis ?
-
 ?
h-coelenterazine + O2 relative activity to native coelenterazine: 12.4% Renilla reniformis ?
-
 ?
h-coelenterazine + O2 relative activity to native coelenterazine: 5.8% Gaussia ?
-
 ?
h-coelenterazine + O2 relative activity to native coelenterazine: 54.1% Renilla reniformis ?
-
 ?
h-coelenterazine + O2 relative activity to native coelenterazine: 68.4% Oplophorus gracilirostris ?
-
 ?
i-coelenterazine + O2 relative activity to native coelenterazine: 0.3% Renilla reniformis ?
-
 ?
i-coelenterazine + O2 relative activity to native coelenterazine: 0.9% Gaussia ?
-
 ?
i-coelenterazine + O2 relative activity to native coelenterazine: 32.3% Oplophorus gracilirostris ?
-
 ?
i-coelenterazine + O2 relative activity to native coelenterazine: 5.1% Renilla reniformis ?
-
 ?
me-coelenterazine + O2 relative activity to native coelenterazine: 4.5% Gaussia ?
-
 ?
me-coelenterazine + O2 relative activity to native coelenterazine: 46.6% Oplophorus gracilirostris ?
-
 ?
me-coelenterazine + O2 relative activity to native coelenterazine: 5.2% Renilla reniformis ?
-
 ?
me-coelenterazine + O2 relative activity to native coelenterazine: 73.6% Renilla reniformis ?
-
 ?
meo-coelenterazine + O2 relative activity to native coelenterazine: 1.2% Renilla reniformis ?
-
 ?
meo-coelenterazine + O2 relative activity to native coelenterazine: 13.6% Gaussia ?
-
 ?
meo-coelenterazine + O2 relative activity to native coelenterazine: 18.2% Renilla reniformis ?
-
 ?
meo-coelenterazine + O2 relative activity to native coelenterazine: 68.1% Oplophorus gracilirostris ?
-
 ?

Synonyms

Synonyms Comment Organism
19kOLase
-
 Oplophorus gracilirostris
GLase
-
 Gaussia
luciferase
-
 Renilla reniformis
luciferase
-
 Oplophorus gracilirostris
luciferase
-
 Gaussia
Oplophorus luciferase
-
 Oplophorus gracilirostris
Renilla luciferase
-
 Renilla reniformis
Renilla luciferase-547 red shift variant Renilla reniformis
RLase
-
 Renilla reniformis
RLase-547 red shift variant Renilla reniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Gaussia
7.6
-
 assay at Renilla reniformis
7.6
-
 assay at Oplophorus gracilirostris