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Literature summary for 1.13.12.5 extracted from
- Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps (2008), Biochem. Biophys. Res. Commun., 365, 96-101.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 0.1 mM | Gaussia princeps |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Gaussia princeps |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| pCold-hGL | two repeat sequences, two catalytic domains | Gaussia princeps |
| pCold-hGL-27/97 | one repeat sequence, with only one catalytic domain | Gaussia princeps |
| pCold-hGL-98/168 | one repeat sequence, with only one catalytic domain | Gaussia princeps |
| pCold-hSGL | with a signal peptide sequence for secretion | Gaussia princeps |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 0.01% | Gaussia princeps | |
| CuCl2 | 1 microM, inhibition of 95% | Gaussia princeps | |
| dithiothreitol | 1 mM | Gaussia princeps | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | insoluble fraction in Escherichia coli cells | Gaussia princeps | - |
- |
| soluble | fraction in Escherichia coli cells | Gaussia princeps | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Br- | stimulates luminescence activity | Gaussia princeps | |
| Cl- | stimulates luminescence activity | Gaussia princeps | |
| Cu2+ | strong inhibition of luminescence activity | Gaussia princeps | |
| I- | stimulates luminescence activity | Gaussia princeps | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10350 | - |
MALDI-TOF-MS analyses, hGL-98/168 | Gaussia princeps |
| 10740 | - |
MALDI-TOF-MS analyses, hGL-27/97 | Gaussia princeps |
| 20020 | - |
MALDI-TOF-MS analyses, hGL | Gaussia princeps |
| 25000 | - |
SDS-PAGE, pCold-hGL | Gaussia princeps |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gaussia princeps | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| souble and insoluble fraction by nickel-chelate column | Gaussia princeps |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1170000000 relative light units/mg, hGL-27/97, soluble fraction | Gaussia princeps |
| additional information | - |
14220000000 relative light units/mg, hGL, insoluble fraction | Gaussia princeps |
| additional information | - |
20000000 relative light units/mg, hGL-98/168, insoluble fraction | Gaussia princeps |
| additional information | - |
3120000000 relative light units/mg, hGL-27/97, insoluble fraction | Gaussia princeps |
| additional information | - |
51230000000 relative light units/mg, hGL, soluble fraction | Gaussia princeps |
| additional information | - |
590000000 relative light units/mg, hGL-98/168, soluble fraction | Gaussia princeps |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| coelenterazine + O2 | 0.5 microg, assay at pH 7.6, high substrate specificity for coelenterazine | Gaussia princeps | coelenteramide + CO2 + hnu | - |
? | |
| cp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| e-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| f-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| fcp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| h-coelenterazine + O2 | assay at pH 7.6m, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| hcp-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| MeO-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
| n-coelenterazine + O2 | assay at pH 7.6, about 100fold reduced relative luminescence | Gaussia princeps | hnu + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gaussia luciferase | - |
Gaussia princeps |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Gaussia princeps |
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