Cloned (Comment) | Organism |
---|---|
expression of stabilized variant RLuc8 with noncleavable N-terminal His6-tag | Renilla reniformis |
Crystallization (Comment) | Organism |
---|---|
free enzyme and in complex with coelenterazine,and mutant K25A/E277A. Diffraction to 1.4 A. Structures demonstrate a classic alpha/beta-hydrolase fold. The presumptive catalytic triad residues are D120, E144, and H285. Additionally determination of structure of the accessory green fluorescent protein | Renilla reniformis |
Protein Variants | Comment | Organism |
---|---|---|
K25A/E277A | surface mutations made with the intention that they would aid in crystallization, not involved in contacts between proteins in the crystal | Renilla reniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
gel filtration | Renilla reniformis |
36800 | - |
1 * 36800, mass spectrometry, 1 * 36900, calculated | Renilla reniformis |
36900 | - |
1 * 36800, mass spectrometry, 1 * 36900, calculated | Renilla reniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Renilla reniformis | P27652 | variant RLuc8 | - |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36800, mass spectrometry, 1 * 36900, calculated | Renilla reniformis |