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Literature summary for 1.13.12.5 extracted from

  • Loening, A.M.; Fenn, T.D.; Gambhir, S.S.
    Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis (2007), J. Mol. Biol., 374, 1017-1028.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of stabilized variant RLuc8 with noncleavable N-terminal His6-tag Renilla reniformis

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with coelenterazine,and mutant K25A/E277A. Diffraction to 1.4 A. Structures demonstrate a classic alpha/beta-hydrolase fold. The presumptive catalytic triad residues are D120, E144, and H285. Additionally determination of structure of the accessory green fluorescent protein Renilla reniformis

Protein Variants

Protein Variants Comment Organism
K25A/E277A surface mutations made with the intention that they would aid in crystallization, not involved in contacts between proteins in the crystal Renilla reniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33800
-
gel filtration Renilla reniformis
36800
-
1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis
36900
-
1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis

Organism

Organism UniProt Comment Textmining
Renilla reniformis P27652 variant RLuc8
-

Subunits

Subunits Comment Organism
monomer 1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis