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Literature summary for 1.13.12.5 extracted from

  • Tsuzuki, K.; Tricoire, L.; Courjean, O.; Gibelin, N.; Rossier, J.; Lambolez, B.
    Thermostable mutants of the photoprotein aequorin obtained by in vitro evolution (2005), J. Biol. Chem., 280, 34324-34331.
    View publication on PubMed
Search for more literature for 1.13.12.5

Protein Variants

Protein Variants Comment Organism
D117G the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 5°C higher than wild-type value Aequorea victoria
D153G the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 7°C higher than wild-type value Aequorea victoria
E128G the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 2°C higher than wild-type value Aequorea victoria
E35G the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is identical to wild-type value Aequorea victoria
F149S the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 5°C lower than wild-type value Aequorea victoria
L170I the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 4°C higher than wild-type value Aequorea victoria
Q168A/L170V the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 8°C higher than wild-type value Aequorea victoria
Q168K/L170V the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 8°C higher than wild-type value Aequorea victoria
Q168R the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 9°C higher than wild-type value Aequorea victoria
Q168R/L170I the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 13°C higher than wild-type value Aequorea victoria
Q168R/L170M the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 8°C higher than wild-type value Aequorea victoria
Q168R/L170V the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 13°C higher than wild-type value Aequorea victoria
V44A the temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock is 1°C higher than wild-type value Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria Q6J4T7
-
-

Synonyms

Synonyms Comment Organism
aequorin
-
 Aequorea victoria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme F149S Aequorea victoria
44
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, wild-type enzyme and mutant enzyme E35G Aequorea victoria
45
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme V44A Aequorea victoria
46
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme E128G Aequorea victoria
48
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme L170I Aequorea victoria
49
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme D117G Aequorea victoria
51
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme D153G Aequorea victoria
52
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme Q168AR/L170M, Q168A/L170V and Q168K/L170V Aequorea victoria
53
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzyme Q168R Aequorea victoria
57
-
 temperature for 50% inactivation of aequorin bioluminescence by a 30-min heat shock, mutant enzymes Q68R/L170I and Q168R/L179V Aequorea victoria