Literature summary for 1.13.12.3 extracted from

Zhang, Y.; Lee, C.; Wehner, N.; Imdahl, F.; Svetlana, V.; Weiste, C.; Droege-Laser, W.; Deeken, R.
Regulation of oncogene expression in T-DNA-transformed host plant cells (2015), PLoS Pathog., 11, 1-27 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box	Agrobacterium tumefaciens

Cloned (Comment)

Organism

gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box

Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan + O2	Agrobacterium tumefaciens	-	(indol-3-yl)acetamide + CO2 + H2O	-	?
L-tryptophan + O2	Agrobacterium tumefaciens C58	-	(indol-3-yl)acetamide + CO2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	Q9R717	-	-
Agrobacterium tumefaciens C58	Q9R717	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + O2	-	Agrobacterium tumefaciens	(indol-3-yl)acetamide + CO2 + H2O	-	?
L-tryptophan + O2	-	Agrobacterium tumefaciens C58	(indol-3-yl)acetamide + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
IaaM	-	Agrobacterium tumefaciens
tryptophan monooxygenase	-	Agrobacterium tumefaciens

Expression

Organism	Comment	Expression
Agrobacterium tumefaciens	gene IaaM is constitutively expressed and no transcription factor further activates its promoter	additional information

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of auxin	Agrobacterium tumefaciens
physiological function	Crown gall development requires the expression of agrobacterial genes in the plant host, e.g. Arabidopsis thaliana. These genes are transferred by the T-DNA of the plant pathogen Agrobacterium tumefaciens and include the oncogenes IaaH, IaaM and Ipt, which, according to the tumor-inducing principle, are essential for crown gall development. Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). The oncogenes are involved in auxin and cytokinin production. The intergenic regions (IGRs) between the coding sequences (CDS) of the three oncogenes function as promoters in plant cells, but gene IaaM is constitutively expressed and no transcription factor further activates its promoter. With increasing auxin levels triggered by ubiquitous expression of IaaH and IaaM, ARF5 is activated and interacts with WRKY40 to potentiate Ipt expression and balance cytokinin and auxin levels for further cell proliferation. Transcriptional regulation of the Agrobacterium tumefaciens genes IaaH, IaaM and Ipt in the host plant, detailed overview	Agrobacterium tumefaciens