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Literature summary for 1.13.12.3 extracted from

  • Mujahid, M.; Sasikala, C.h.; Ramana, C.h.V.
    Aniline is an inducer, and not a precursor, for indole derivatives in Rubrivivax benzoatilyticus JA2 (2014), PLoS ONE, 9, e87503 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
quantitative real time RT-PCR enzyme expression analysis Rubrivivax benzoatilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + O2 Rubrivivax benzoatilyticus
-
(indol-3-yl)acetamide + CO2 + H2O
-
?
L-tryptophan + O2 Rubrivivax benzoatilyticus JA2
-
(indol-3-yl)acetamide + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rubrivivax benzoatilyticus
-
-
-
Rubrivivax benzoatilyticus JA2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + O2
-
Rubrivivax benzoatilyticus (indol-3-yl)acetamide + CO2 + H2O
-
?
L-tryptophan + O2
-
Rubrivivax benzoatilyticus JA2 (indol-3-yl)acetamide + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
TMO
-
Rubrivivax benzoatilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rubrivivax benzoatilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Rubrivivax benzoatilyticus

Expression

Organism Comment Expression
Rubrivivax benzoatilyticus aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway, including tryptophan 2-monooxygenase activity, by possibly modulating the metabolic pathway up

General Information

General Information Comment Organism
metabolism tryptophan aminotransferase (TAT) and tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Indole biosynthesis in strain JA2 occurs via the de novo shikimate pathway. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborates well with indole 3-acetic acid levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway Rubrivivax benzoatilyticus
physiological function tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Metabolic profiling reveals tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid and indole 3-aldehyde, the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies reveal that while fumarate is the precursor of indole biosynthesis, aniline is not a precursor of indoles in strain JA2, but is an indole biosynthesis stimulator Rubrivivax benzoatilyticus