Cloned (Comment) | Organism |
---|---|
quantitative real time RT-PCR enzyme expression analysis | Rubrivivax benzoatilyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | Rubrivivax benzoatilyticus | - |
(indol-3-yl)acetamide + CO2 + H2O | - |
? | |
L-tryptophan + O2 | Rubrivivax benzoatilyticus JA2 | - |
(indol-3-yl)acetamide + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrivivax benzoatilyticus | - |
- |
- |
Rubrivivax benzoatilyticus JA2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | - |
Rubrivivax benzoatilyticus | (indol-3-yl)acetamide + CO2 + H2O | - |
? | |
L-tryptophan + O2 | - |
Rubrivivax benzoatilyticus JA2 | (indol-3-yl)acetamide + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TMO | - |
Rubrivivax benzoatilyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rubrivivax benzoatilyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rubrivivax benzoatilyticus |
Organism | Comment | Expression |
---|---|---|
Rubrivivax benzoatilyticus | aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway, including tryptophan 2-monooxygenase activity, by possibly modulating the metabolic pathway | up |
General Information | Comment | Organism |
---|---|---|
metabolism | tryptophan aminotransferase (TAT) and tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Indole biosynthesis in strain JA2 occurs via the de novo shikimate pathway. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborates well with indole 3-acetic acid levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway | Rubrivivax benzoatilyticus |
physiological function | tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Metabolic profiling reveals tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid and indole 3-aldehyde, the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies reveal that while fumarate is the precursor of indole biosynthesis, aniline is not a precursor of indoles in strain JA2, but is an indole biosynthesis stimulator | Rubrivivax benzoatilyticus |