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Literature summary for 1.13.12.3 extracted from

  • Sobrado, P.; Fitzpatrick, P.F.
    Identification of Tyr413 as an active site residue in the flavoprotein tryptophan 2-monooxygenase and analysis of its contribution to catalysis (2003), Biochemistry, 42, 13833-13838.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
Y413A 200fold reduction in first-order rate constant for reduction, 33fold decrease in rate constant for oxidation, conversion of enzyme to oxidase Pseudomonas savastanoi
Y413F 35fold reduction in first-order rate constant for reduction, 11fold decrease in rate constant for oxidation, conversion of enzyme to oxidase Pseudomonas savastanoi

Inhibitors

Inhibitors Comment Organism Structure
Indole-3-acetamide
-
Pseudomonas savastanoi
Indole-3-pyruvate
-
Pseudomonas savastanoi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
L-tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi
0.09
-
O2 pH 8.3, 25°C, wild-type Pseudomonas savastanoi
0.12
-
O2 pH 8.3, 25°C, mutant Y413A Pseudomonas savastanoi
0.22
-
O2 pH 8.3, 25°C, mutant Y413F Pseudomonas savastanoi
1
-
L-tryptophan pH 8.3, 25°C, mutant Y413F Pseudomonas savastanoi
1.48
-
L-tryptophan pH 8.3, 25°C, mutant Y413A Pseudomonas savastanoi

Organism

Organism UniProt Comment Textmining
Pseudomonas savastanoi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + O2
-
Pseudomonas savastanoi indole-3-acetamide + CO2 + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
L-tryptophan pH 8.3, 25°C, mutant Y413A Pseudomonas savastanoi
4
-
L-tryptophan pH 8.3, 25°C, mutant Y413F Pseudomonas savastanoi
13.2
-
L-tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.69
-
Indole-3-pyruvate pH 8.3, 25°C, mutant Y413A Pseudomonas savastanoi
0.69
-
Indole-3-acetamide pH 8.3, 25°C, mutant Y413A Pseudomonas savastanoi
4
-
Indole-3-pyruvate pH 8.3, 25°C, mutant Y413F Pseudomonas savastanoi
4
-
Indole-3-acetamide pH 8.3, 25°C, mutant Y413F Pseudomonas savastanoi
13.2
-
Indole-3-pyruvate pH 8.3, 25°C, wild-type Pseudomonas savastanoi
13.2
-
Indole-3-acetamide pH 8.3, 25°C, wild-type Pseudomonas savastanoi