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Literature summary for 1.13.12.3 extracted from
- Purification and characterization of the flavoprotein tryptophan 2-monooxygenase expressed at high levels in Escherichia coli (1995), Arch. Biochem. Biophys., 316, 241-248.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas savastanoi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-methyl methanethiolsulfonate | - |
Pseudomonas savastanoi |  |
| D-methionine | competitive inhibition | Pseudomonas savastanoi | |
| D-phenylalanine | competitive inhibition | Pseudomonas savastanoi | |
| D-tryptophan | competitive inhibitor | Pseudomonas savastanoi | |
| diethylpyrocarbonate | - |
Pseudomonas savastanoi | |
| diethylthiocarbonate | - |
Pseudomonas savastanoi | |
| Hydrocinnamic acid | competitive inhibition | Pseudomonas savastanoi | |
| Indole-3-acetamide | - |
Pseudomonas savastanoi | |
| indole-3-acetic acid | - |
Pseudomonas savastanoi | |
| N-ethylmaleimide | - |
Pseudomonas savastanoi | |
| phenylacetamide | competitive inhibition | Pseudomonas savastanoi | |
| Phenylethylamine | competitive inhibition | Pseudomonas savastanoi | |
| phenyllactic acid | competitive inhibition | Pseudomonas savastanoi | |
| phenylpyruvic acid | competitive inhibition | Pseudomonas savastanoi | |
| tryptamine | competitive inhibition | Pseudomonas savastanoi | |
| Vinylglycine | - |
Pseudomonas savastanoi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
L-tryptophan | - |
Pseudomonas savastanoi | |
| 1.3 | - |
D,L-3-phenyl-2-hydrazinopropanoic acid | - |
Pseudomonas savastanoi | |
| 2.4 | - |
L-phenylalanine | - |
Pseudomonas savastanoi | |
| 22.4 | - |
L-methionine | - |
Pseudomonas savastanoi | |
| 22.6 | - |
L-alanine | - |
Pseudomonas savastanoi | |
| 22.6 | - |
D,L-N-methyl-phenylalanine | - |
Pseudomonas savastanoi | |
| 32 | - |
D,L-3-pyridylalanine | - |
Pseudomonas savastanoi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + O2 | Pseudomonas savastanoi | - |
indole-3-acetamide + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas savastanoi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas savastanoi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.7 | - |
- |
Pseudomonas savastanoi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several weeks without significant loss in activity | Pseudomonas savastanoi |
| 4°C, several weeks without significant loss in activity | Pseudomonas savastanoi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,L-3-phenyl-2-hydrazinopropanoic acid + O2 | - |
Pseudomonas savastanoi | 2-phenylacetohydrazide + CO2 + H2O | - |
? | |
| D,L-3-pyridylalanine + O2 | - |
Pseudomonas savastanoi | 2-pyridylacetamide + CO2 + H2O | - |
? | |
| D,L-N-methyl-phenylalanine + O2 | - |
Pseudomonas savastanoi | N-methyl-2-phenylacetamide + CO2 + H2O | - |
? | |
| L-alanine + O2 | - |
Pseudomonas savastanoi | acetamide + CO2 + H2O | - |
? | |
| L-methionine + O2 | - |
Pseudomonas savastanoi | 3-(methylthio)propanamide + CO2 + H2O | - |
? | |
| L-phenylalanine + O2 | - |
Pseudomonas savastanoi | phenylacetamide + CO2 + H2O | - |
? | |
| L-tryptophan + O2 | - |
Pseudomonas savastanoi | indole-3-acetamide + CO2 + H2O | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Pseudomonas savastanoi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000167 | - |
D,L-3-phenyl-2-hydrazinopropanoic acid | - |
Pseudomonas savastanoi | |
| 0.000317 | - |
L-alanine | - |
Pseudomonas savastanoi | |
| 0.000317 | - |
D,L-N-methyl-phenylalanine | - |
Pseudomonas savastanoi | |
| 0.00075 | - |
D,L-3-pyridylalanine | - |
Pseudomonas savastanoi | |
| 0.00155 | - |
L-methionine | - |
Pseudomonas savastanoi | |
| 0.00305 | - |
L-phenylalanine | - |
Pseudomonas savastanoi | |
| 0.00367 | - |
L-tryptophan | - |
Pseudomonas savastanoi | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 11 | - |
Pseudomonas savastanoi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas savastanoi | |
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