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Literature summary for 1.13.12.3 extracted from

  • Kosuge, T.; Heskett, M.G.; Wilson, E.E.
    Microbial synthesis and degradation of indole-3-acetic acid. I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi (1966), J. Biol. Chem., 241, 3738-3744.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol weak Pseudomonas savastanoi
BaCl2 weak Pseudomonas savastanoi
CaCl2 little or no effect Pseudomonas savastanoi
CuCl2
-
Pseudomonas savastanoi
D-tryptophan weak Pseudomonas savastanoi
EDTA little or no effect Pseudomonas savastanoi
HgCl2
-
Pseudomonas savastanoi
hydroxylamine
-
Pseudomonas savastanoi
Indole-3-acetaldehyde weak Pseudomonas savastanoi
Indole-3-acetamide end product inhibition Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
iodoacetamide
-
Pseudomonas savastanoi
isoniazid
-
Pseudomonas savastanoi
K3Fe(CN)6 weak Pseudomonas savastanoi
KCN little or no effect Pseudomonas savastanoi
L-cysteine
-
Pseudomonas savastanoi
L-glutathione reduced Pseudomonas savastanoi
Quinacrine weak Pseudomonas savastanoi
Semicarbazide
-
Pseudomonas savastanoi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + O2 Pseudomonas savastanoi specific for L-isomer of tryptophan indole-3-acetamide + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas savastanoi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas savastanoi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
-
Pseudomonas savastanoi

Storage Stability

Storage Stability Organism
-10°C, protein concentration 4 mg/ml, 2 months, less than 10% loss of activity Pseudomonas savastanoi
-10°C, protein concentration less than 0.3 mg/ml, 40% loss of activity after 24 h Pseudomonas savastanoi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxytryptophan + O2 at 17% the rate of that for L-tryptophan Pseudomonas savastanoi 5-hydroxyindole-3-acetamide + CO2 + H2O
-
?
L-phenylalanine + O2 weak activity Pseudomonas savastanoi phenylacetamide + CO2 + H2O
-
?
L-tryptophan + O2 specific for L-isomer of tryptophan Pseudomonas savastanoi indole-3-acetamide + CO2 + H2O
-
?
L-tyrosine + O2 weak activity Pseudomonas savastanoi 4-hydroxyphenylacetamide + CO2 + H2O
-
?
additional information no activity with: indole-3-pyruvate, indole-3-acetaldehyde, alpha-methyltryptophan, indole-3-acetic acid Pseudomonas savastanoi ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas savastanoi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 7.4, protein concentration above 4 mg/ml, 2 min, stable, 40% loss of activity after 5 min Pseudomonas savastanoi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9.2
-
Pseudomonas savastanoi

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 pH 5.5: about 50% of activity maximum, pH 10: about 55% of activity maximum Pseudomonas savastanoi