BRENDA - Enzyme Database
show all sequences of 1.13.12.24

Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea

Shimomura, O.; Johnson, F.H.; Saiga, Y.; J. Cell. Comp. Physiol. 59, 223-239 (1962)

Data extracted from this reference:

General Stability
General Stability
Organism
protein is relatively stable in 0.01 M Na2EDTA
Aequorea aequorea
Inhibitors
Inhibitors
Commentary
Organism
Structure
Barbiturate
50 mM, complete loss of activity
Aequorea aequorea
Cyanate
50 mM, complete loss of activity
Aequorea aequorea
EDTA
-
Aequorea aequorea
glutathione
10 mM, complete loss of activity
Aequorea aequorea
H2O2
50 mM, complete loss of activity
Aequorea aequorea
malonate
50 mM, complete loss of activity
Aequorea aequorea
NaAsO2
20 mM, complete loss of activity
Aequorea aequorea
nitrite
50 mM, complete loss of activity
Aequorea aequorea
thiocyanate
50 mM, complete loss of activity
Aequorea aequorea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required for luminescence reaction
Aequorea aequorea
Organic Solvent Stability
Organic Solvent
Commentary
Organism
benzyl alcohol
50 mM, 10% increase in activity
Aequorea aequorea
n-amyl alcohol
50 mM, 7% increase in activity
Aequorea aequorea
n-Butanol
50 mM, 8% increase in activity
Aequorea aequorea
n-heptanol
50 mM, 30% increase in activity
Aequorea aequorea
n-hexanol
50 mM, 10% increase in activity
Aequorea aequorea
n-octanol
50 mM, 18% increase in activity
Aequorea aequorea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aequorea aequorea
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aequorea aequorea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1
8.3
-
Aequorea aequorea
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
unstable below
Aequorea aequorea
General Stability (protein specific)
General Stability
Organism
protein is relatively stable in 0.01 M Na2EDTA
Aequorea aequorea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Barbiturate
50 mM, complete loss of activity
Aequorea aequorea
Cyanate
50 mM, complete loss of activity
Aequorea aequorea
EDTA
-
Aequorea aequorea
glutathione
10 mM, complete loss of activity
Aequorea aequorea
H2O2
50 mM, complete loss of activity
Aequorea aequorea
malonate
50 mM, complete loss of activity
Aequorea aequorea
NaAsO2
20 mM, complete loss of activity
Aequorea aequorea
nitrite
50 mM, complete loss of activity
Aequorea aequorea
thiocyanate
50 mM, complete loss of activity
Aequorea aequorea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required for luminescence reaction
Aequorea aequorea
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
benzyl alcohol
50 mM, 10% increase in activity
Aequorea aequorea
n-amyl alcohol
50 mM, 7% increase in activity
Aequorea aequorea
n-Butanol
50 mM, 8% increase in activity
Aequorea aequorea
n-heptanol
50 mM, 30% increase in activity
Aequorea aequorea
n-hexanol
50 mM, 10% increase in activity
Aequorea aequorea
n-octanol
50 mM, 18% increase in activity
Aequorea aequorea
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aequorea aequorea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1
8.3
-
Aequorea aequorea
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
unstable below
Aequorea aequorea
Other publictions for EC 1.13.12.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745608
Eremeeva
Bioluminescent and biochemica ...
Aequorea victoria, Obelia longissima
J. Photochem. Photobiol. B
174
97-105
2017
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746377
Inouye
A fusion protein of the synth ...
Aequorea victoria
Protein Expr. Purif.
137
58-63
2017
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745241
Inouye
Expression and characterizati ...
Aequorea victoria
J. Biochem.
160
59-68
2016
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746238
Bakayan
Red fluorescent protein-aequo ...
Aequorea victoria
PLoS ONE
6
e19520
2011
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744479
Doi
Selective protein modificatio ...
Aequorea victoria
Bioorg. Med. Chem.
17
3399-3404
2009
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744216
Inouye
Imidazole-assisted catalysis ...
Aequorea victoria
Biochem. Biophys. Res. Commun.
354
650-655
2007
2
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744892
Inouye
Blue fluorescent protein from ...
Aequorea victoria
FEBS Lett.
580
1977-1982
2006
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744890
Stepanyuk
Interchange of aequorin and o ...
Aequorea victoria, Obelia longissima
FEBS Lett.
579
1008-1014
2005
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745232
Ohashi
NMR analysis of the Mg2+-bind ...
Aequorea victoria
J. Biochem.
138
613-620
2005
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745276
Tsuzuki
Thermostable mutants of the p ...
Aequorea victoria
J. Biol. Chem.
280
34324-34331
2005
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746389
Deng
All three Ca2+-binding loops ...
Aequorea victoria, Obelia longissima
Protein Sci.
14
663-675
2005
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744889
Inouye
Blue fluorescent protein from ...
Aequorea victoria
FEBS Lett.
577
105-110
2004
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746382
Glynou
One-step purification and ref ...
Aequorea victoria
Protein Expr. Purif.
27
384-390
2003
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745878
Head
The crystal structure of the ...
Aequorea victoria
Nature
405
372-376
2000
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744262
Watkins
Requirement of the C-terminal ...
Aequorea victoria
Biochem. J.
293
181-185
1993
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744887
Ohmiya
Bioluminescence of the Ca(2+) ...
Aequorea victoria
FEBS Lett.
320
267-270
1993
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744205
Kendall
Engineering the Ca2+-activate ...
Aequorea victoria
Biochem. Biophys. Res. Commun.
187
1091-1097
1992
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744204
Nakajima-Shimada
Galactose-dependent expressio ...
Aequorea victoria
Biochem. Biophys. Res. Commun.
174
115-122
1991
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746328
Kurose
Bioluminescence of the Ca2+-b ...
Aequorea victoria
Proc. Natl. Acad. Sci. USA
86
80-84
1989
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746326
Inouye
Cloning and sequence analysis ...
Aequorea victoria
Proc. Natl. Acad. Sci. USA
82
3154-3158
1985
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746325
Shimomura
Peroxidized coelenterazine, t ...
Aequorea victoria
Proc. Natl. Acad. Sci. USA
75
2611-2615
1978
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744286
Hori
Renilla luciferin as the subs ...
Aequorea victoria
Biochemistry
14
2371-2376
1975
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744285
Morise
Intermolecular energy transfe ...
Aequorea victoria
Biochemistry
13
2656-2662
1974
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745437
Shimomura
Extraction, purification and ...
Aequorea aequorea
J. Cell. Comp. Physiol.
59
223-239
1962
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