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Literature summary for 1.13.12.24 extracted from

  • Tsuzuki, K.; Tricoire, L.; Courjean, O.; Gibelin, N.; Rossier, J.; Lambolez, B.
    Thermostable mutants of the photoprotein aequorin obtained by in vitro evolution (2005), J. Biol. Chem., 280, 34324-34331 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F149 residue participates in stabilization of the coelenterazine peroxide and the triggering of photon emission by linking the third EF-hand to Trp-129 and His-169 coelenterazine binding residues Aequorea victoria
H27N 18fold increase of wild-type activity Aequorea victoria
H27N/Q168R 13fold increase of wild-type activity Aequorea victoria
K17R 13fold increase of wild-type activity Aequorea victoria
K17R/H27N/Q168R 11fold increase of wild-type activity Aequorea victoria
K17R/L170I 8fold increase of wild-type activity Aequorea victoria
K17R/N26D 14fold increase of wild-type activity Aequorea victoria
K17R/N26D/Q168R 14fold increase of wild-type activity Aequorea victoria
K17R/Q168R 25fold increase of wild-type activity Aequorea victoria
L170I 16fold increase of wild-type activity, an increase of the photoprotein lifetime at 37°C and increased thermostability Aequorea victoria
additional information mutations with increased luminescence intensity neighbor the His-16 or His-169 coelenterazine binding residues or are located in the first EF-hand Aequorea victoria
N26D 13fold increase of wild-type activity Aequorea victoria
N26D/Q168R 8fold increase of wild-type activity Aequorea victoria
Q168 residue participates in stabilization of the coelenterazine peroxide and the triggering of photon emission by linking the third EF-hand to Trp-129 and His-169 coelenterazine binding residues Aequorea victoria
Q168R 11fold increase of wild-type activity, increase of the photoprotein lifetime at 37°C and increased thermostablility Aequorea victoria
Q168R/L170I mutant displays increased thermostablility Aequorea victoria
V25A 10fold increase of wild-type activity Aequorea victoria
V25I/L170I 24fold increase of wild-type activity Aequorea victoria
V25I/Q168R 20fold increase of wild-type activity Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria Q6J4T7
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Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
wild-type, 50% inactivation of aequorin bioluminescence by a 30-min heat shock Aequorea victoria
48
-
mutant L170I, 50% inactivation of aequorin bioluminescence by a 30-min heat shock Aequorea victoria
53
-
mutant Q168R, 50% inactivation of aequorin bioluminescence by a 30-min heat shock Aequorea victoria
57
-
mutant Q168R/L170I, 50% inactivation of aequorin bioluminescence by a 30-min heat shock Aequorea victoria