BRENDA - Enzyme Database
show all sequences of 1.13.12.23

CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis

Pojer, F.; Kahlich, R.; Kammerer, B.; Li, S.M.; Heide, L.; J. Biol. Chem. 278, 30661-30668 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme is not stimulated by addition of 2-oxoglutarate
Streptomyces roseochromogenes
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces roseochromogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity
Streptomyces roseochromogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-dimethylallyl-4-hydroxymandelate + O2
Streptomyces roseochromogenes
-
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
?
3-dimethylallyl-4-hydroxymandelate + O2
Streptomyces roseochromogenes DS 12976
-
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces roseochromogenes
Q8GHB1
-
-
Streptomyces roseochromogenes DS 12976
Q8GHB1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
glutathione-Sepharose column chromatography
Streptomyces roseochromogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-dimethylallyl-4-hydroxymandelate + O2
-
742826
Streptomyces roseochromogenes
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
-
?
3-dimethylallyl-4-hydroxymandelate + O2
-
742826
Streptomyces roseochromogenes DS 12976
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 30500, calculated from amino acid sequence
Streptomyces roseochromogenes
Synonyms
Synonyms
Commentary
Organism
CloR
-
Streptomyces roseochromogenes
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme is not stimulated by addition of 2-oxoglutarate
Streptomyces roseochromogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces roseochromogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity
Streptomyces roseochromogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-dimethylallyl-4-hydroxymandelate + O2
Streptomyces roseochromogenes
-
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
?
3-dimethylallyl-4-hydroxymandelate + O2
Streptomyces roseochromogenes DS 12976
-
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione-Sepharose column chromatography
Streptomyces roseochromogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-dimethylallyl-4-hydroxymandelate + O2
-
742826
Streptomyces roseochromogenes
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
-
?
3-dimethylallyl-4-hydroxymandelate + O2
-
742826
Streptomyces roseochromogenes DS 12976
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30500, calculated from amino acid sequence
Streptomyces roseochromogenes
Other publictions for EC 1.13.12.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742826
Pojer
CloR, a bifunctional non-heme ...
Streptomyces roseochromogenes, Streptomyces roseochromogenes DS 12976
J. Biol. Chem.
278
30661-30668
2003
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743244
Pojer
Molecular cloning and sequenc ...
Streptomyces roseochromogenus subsp. oscitans, Streptomyces roseochromogenus subsp. oscitans DS 12.976
Microbiology
148
3901-3911
2002
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