BRENDA - Enzyme Database
show all sequences of 1.13.12.21

Tetracenomycin F1 monooxygenase: oxidation of a naphthacenone to a naphthacenequinone in the biosynthesis of tetracenomycin C in Streptomyces glaucescens

Shen, B.; Hutchinson, C.R.; Biochemistry 32, 6656-6663 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CuCl2
31% loss of activity
Streptomyces glaucescens
diethyl dicarbonate
-
Streptomyces glaucescens
N-ethylmaleimide
0.025 mM, complete inhibition
Streptomyces glaucescens
p-chloromercuribenzoate
0.025 mM, complete inhibition
Streptomyces glaucescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00747
-
tetracenomycin F1
pH 7.5, 30°C
Streptomyces glaucescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
does not use a metal ion
Streptomyces glaucescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12500
-
3 * 12500, SDS-PAGE
Streptomyces glaucescens
37500
-
gel filtration
Streptomyces glaucescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetracenomycin F1 + O2
Streptomyces glaucescens
the enzyme is involved in the biosynthesis of tetracenomycin C
tetracenomycin D3 + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces glaucescens
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces glaucescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.599
-
pH 7.5, 30°C
Streptomyces glaucescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
tetracenomycin F1 + O2
the enzyme is involved in the biosynthesis of tetracenomycin C
721576
Streptomyces glaucescens
tetracenomycin D3 + H2O
-
-
-
?
tetracenomycin F1 + O2
i.e. 3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate = 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-2-naphthacenecarboxylic acid
721576
Streptomyces glaucescens
tetracenomycin D3 + H2O
i.e. 3,8,10,12-tetrahydroxy-1-methyl-11-oxo-6,11-dihydro-2-tetracenecarboxylate, i.e. 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-11-oxo-2-naphthacenecarboxylic acid
-
-
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 12500, SDS-PAGE
Streptomyces glaucescens
Synonyms
Synonyms
Commentary
Organism
TcmF1 monooxygenase
-
Streptomyces glaucescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces glaucescens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
pH 7.0: about 40% of maximal activity, pH 8.0: about 85% of maximal activity
Streptomyces glaucescens
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
does not possess a prosthetic group such as flavin or heme
Streptomyces glaucescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
does not possess a prosthetic group such as flavin or heme
Streptomyces glaucescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuCl2
31% loss of activity
Streptomyces glaucescens
diethyl dicarbonate
-
Streptomyces glaucescens
N-ethylmaleimide
0.025 mM, complete inhibition
Streptomyces glaucescens
p-chloromercuribenzoate
0.025 mM, complete inhibition
Streptomyces glaucescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00747
-
tetracenomycin F1
pH 7.5, 30°C
Streptomyces glaucescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
does not use a metal ion
Streptomyces glaucescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12500
-
3 * 12500, SDS-PAGE
Streptomyces glaucescens
37500
-
gel filtration
Streptomyces glaucescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetracenomycin F1 + O2
Streptomyces glaucescens
the enzyme is involved in the biosynthesis of tetracenomycin C
tetracenomycin D3 + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces glaucescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.599
-
pH 7.5, 30°C
Streptomyces glaucescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
tetracenomycin F1 + O2
the enzyme is involved in the biosynthesis of tetracenomycin C
721576
Streptomyces glaucescens
tetracenomycin D3 + H2O
-
-
-
?
tetracenomycin F1 + O2
i.e. 3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate = 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-2-naphthacenecarboxylic acid
721576
Streptomyces glaucescens
tetracenomycin D3 + H2O
i.e. 3,8,10,12-tetrahydroxy-1-methyl-11-oxo-6,11-dihydro-2-tetracenecarboxylate, i.e. 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-11-oxo-2-naphthacenecarboxylic acid
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 12500, SDS-PAGE
Streptomyces glaucescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces glaucescens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
pH 7.0: about 40% of maximal activity, pH 8.0: about 85% of maximal activity
Streptomyces glaucescens
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of tetracenomycin C
Streptomyces glaucescens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of tetracenomycin C
Streptomyces glaucescens
Other publictions for EC 1.13.12.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721576
Shen
Tetracenomycin F1 monooxygenas ...
Streptomyces glaucescens
Biochemistry
32
6656-6663
1993
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4
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2
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2
1
1
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1
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1
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4
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1
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1
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1
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1
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2
1
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1
1
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1
1
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722482
Summers
The tcmVI region of the tetrac ...
Streptomyces glaucescens
J. Bacteriol.
175
7571-7580
1993
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1
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