Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 31% loss of activity | Streptomyces glaucescens | |
diethyl dicarbonate | - |
Streptomyces glaucescens | |
N-ethylmaleimide | 0.025 mM, complete inhibition | Streptomyces glaucescens | |
p-chloromercuribenzoate | 0.025 mM, complete inhibition | Streptomyces glaucescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00747 | - |
tetracenomycin F1 | pH 7.5, 30°C | Streptomyces glaucescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not use a metal ion | Streptomyces glaucescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12500 | - |
3 * 12500, SDS-PAGE | Streptomyces glaucescens |
37500 | - |
gel filtration | Streptomyces glaucescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetracenomycin F1 + O2 | Streptomyces glaucescens | the enzyme is involved in the biosynthesis of tetracenomycin C | tetracenomycin D3 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces glaucescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces glaucescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.599 | - |
pH 7.5, 30°C | Streptomyces glaucescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetracenomycin F1 + O2 | the enzyme is involved in the biosynthesis of tetracenomycin C | Streptomyces glaucescens | tetracenomycin D3 + H2O | - |
? | |
tetracenomycin F1 + O2 | i.e. 3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate = 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-2-naphthacenecarboxylic acid | Streptomyces glaucescens | tetracenomycin D3 + H2O | i.e. 3,8,10,12-tetrahydroxy-1-methyl-11-oxo-6,11-dihydro-2-tetracenecarboxylate, i.e. 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-11-oxo-2-naphthacenecarboxylic acid | ? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 12500, SDS-PAGE | Streptomyces glaucescens |
Synonyms | Comment | Organism |
---|---|---|
TcmF1 monooxygenase | - |
Streptomyces glaucescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces glaucescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | pH 7.0: about 40% of maximal activity, pH 8.0: about 85% of maximal activity | Streptomyces glaucescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | does not possess a prosthetic group such as flavin or heme | Streptomyces glaucescens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of tetracenomycin C | Streptomyces glaucescens |