Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for production of 5-aminovalerate, a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli is engineered for production of 5-aminovalerate from L-lysine by coupled reaction of recombinant DavB, L-lysine monooxygenase, and recombinant DavA, 5-aminovaleramidase. Because L-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene davB, recombinant expression of His-tagged enzyme DavB in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Escherichia coli is engineered for production of 5-aminovalerate from L-lysine by coupled reaction of recombinant DavB, L-lysine monooxygenase, and recombinant DavA, 5-aminovaleramidase, overview. Under optimal conditions, 20.8 g/l 5-aminovalerate is produced from 30 g/l L-lysine in 12 h. Hydrogen peroxide, which is produced during the process of L-lysine oxidization, will further oxidize 6-amino-2-ketocaproic acid to form 5-aminovalerate as the final product. Method optimization, overview | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 | Pseudomonas putida | - |
5-aminopentanamide + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88QV1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme DavB from Escherichia coli by ncikel affinity chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.1 | - |
purified recombinant enzyme, pH 7.0, 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 | - |
Pseudomonas putida | 5-aminopentanamide + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62400, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
davB | - |
Pseudomonas putida |
L-lysine monooxygenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | DavB is a FAD-dependent monooxygenase | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | L-lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the 5-aminovalerate pathway of various microorganisms. DavB catalyzes the oxidation of L-lysine to produce 5-aminovaleramide. DavA then catalyzes 5-aminovaleramide into 5-aminovalerate | Pseudomonas putida |
physiological function | the enzyme produces 5-aminovalerate, a metabolite of L-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA, EC 3.5.1.30) play key roles in the biotransformation of L-lysine into 5-aminovalerate | Pseudomonas putida |