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Literature summary for 1.13.12.2 extracted from

  • Shin, J.H.; Park, S.H.; Oh, Y.H.; Choi, J.W.; Lee, M.H.; Cho, J.S.; Jeong, K.J.; Joo, J.C.; Yu, J.; Park, S.J.; Lee, S.Y.
    Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid (2016), Microb. Cell Fact., 15, 174 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.12.2

Cloned(Commentary)

Cloned (Comment) Organism
gene davB, recombinant expression in Escherichia coli, coexpression with gene davA, encoding 5-aminovaleramidase, recombinant expression of gene davB as N-terminally His-tagged enzyme in Corynebacterium glutamicum, a highly L-lysine producing strain BE using the sod promoter and the transcription factor Tu, EC 3.6.5.3 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information engineering of a recombinant Escherichia coli strain expressing the davB and davA genes for bioconversion of L-lysine to 5-aminovaleric acid resulting in low levels of 5-aminovalerate. Development of metabolically engineered Corynebacterium glutamicum strains for enhanced fermentative production of 5-aminovalerate from glucose. Expression of the Corynebacterium glutamicum codon-optimized davA gene fused with His6-Tag at its N-terminus and the davB gene as an operon under a strong synthetic H36 promoter (plasmid p36davAB3) in Corynebacterium glutamicum strain BE (pJS38) enables the most efficient production of 5-aminovalerate. The construct containing the His6-tagged variant produces substantially more 5-aminovalerate compared to that produced using the construct lacking the His-tag, possibly because of the improved stability afforded by the 5'-modification, which results in higher expression of the davAB genes in the recombinant Corynebacterium glutamicum BE strain. Deletion of the gabT gene (EC 2.6.1.19), encoding 4-aminobutyrate aminotransferase, improves the 5-aminovalerate production Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 Pseudomonas putida
-
 5-aminopentanamide + CO2 + H2O
-
 ?
L-lysine + O2 Pseudomonas putida ATCC 12633
-
 5-aminopentanamide + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ATCC 12633
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2
-
 Pseudomonas putida 5-aminopentanamide + CO2 + H2O
-
 ?
L-lysine + O2
-
 Pseudomonas putida ATCC 12633 5-aminopentanamide + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
davB
-
 Pseudomonas putida
L-lysine 2-monooxygenase
-
 Pseudomonas putida

General Information

General Information Comment Organism
metabolism the enzyme is involved in the aminovalerate pathway, overview Pseudomonas putida