Cloned (Comment) | Organism |
---|---|
histidine-tagged Lingulodinium polyedrum luciferase domain 3 is expressed in Escherichia coli BL21(DE3) cells | Lingulodinium polyedra |
Crystallization (Comment) | Organism |
---|---|
Lingulodinium polyedrum luciferase domain 3, vapor diffusion method, using 18-20% (w/v) polyethylene glycol 2000 methyl ether and 100 mM (2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (pH 8.0) | Lingulodinium polyedra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lingulodinium polyedra | each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lingulodinium polyedra | O77206 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel metal chelate column chromatography | Lingulodinium polyedra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
additional information | each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light | Lingulodinium polyedra | ? | - |
? |