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Literature summary for 1.13.12.16 extracted from
- Kinetic solvent viscosity effects reveal a protein isomerization in the reductive half-reaction of Neurospora crassa class II nitronate monooxygenase (2020), Arch. Biochem. Biophys., 695, 108625 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
O2 | Km less or equal 0.001 mM, with 3-nitropropionic acid as cosubstrate, at pH 7.4 and 30°C | Neurospora crassa |  |
| 0.009 | - |
O2 | Km less or equal 0.009 mM, with propionate 3-nitronate as cosubstrate, at pH 7.4 and 30°C | Neurospora crassa | |
| 0.15 | - |
propionate 3-nitronate | at pH 7.4 and 30°C | Neurospora crassa | |
| 12 | - |
3-nitropropionic acid | at pH 7.4 and 30°C | Neurospora crassa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl Sepharose column chromatography and DEAE Sepharose column chromatography | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-nitropropionic acid + O2 | - |
Neurospora crassa | ? | - |
? | |
| propionate 3-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NMO | - |
Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42 | - |
3-nitropropionic acid | at pH 7.4 and 30°C | Neurospora crassa | |
| 300 | - |
propionate 3-nitronate | at pH 7.4 and 30°C | Neurospora crassa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | dependent on | Neurospora crassa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
3-nitropropionic acid | at pH 7.4 and 30°C | Neurospora crassa | |
| 2000 | - |
propionate 3-nitronate | at pH 7.4 and 30°C | Neurospora crassa | |
| 33000 | - |
O2 | kcat_Km at least 33000 1/sec*mM with propionate 3-nitronate as cosubstrate, at pH 7.4 and 30°C | Neurospora crassa | |
| 42000 | - |
O2 | kcat_Km at least 42000 1/sec*mM, with 3-nitropropionic acid as cosubstrate, at pH 7.4 and 30°C | Neurospora crassa | |
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Release 2023.1 (January 2023)
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