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Literature summary for 1.13.12.16 extracted from

  • Cervantes Quintero, K.Y.; Padilla Guerrero, I.E.; Torres Guzman, J.C.; Villa Martinez, B.G.; Valencia Felix, A.; Gonzalez Hernandez, G.A.
    Members of the nitronate monooxygenase gene family from Metarhizium brunneum are induced during the process of infection to Plutella xylostella (2020), Appl. Microbiol. Biotechnol., 104, 2987-2997 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Metarhizium brunneum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.3
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.6
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
2.8
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
3.4
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
4.32
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
5.6
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
10.6
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
12.9
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
13.6
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
20.1
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
34.3
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
45.1
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
50.6
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
91.4
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
134.9
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
147.8
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
148.5
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum

Organism

Organism UniProt Comment Textmining
Metarhizium brunneum
-
-
-
Metarhizium brunneum A0A6C0WVH0
-
-
Metarhizium brunneum A0A6C0WVK2
-
-
Metarhizium brunneum A0A6C0WW14
-
-
Metarhizium brunneum A0A6C0WWH2
-
-
Metarhizium brunneum Ma10
-
-
-
Metarhizium brunneum Ma10 A0A6C0WVH0
-
-
Metarhizium brunneum Ma10 A0A6C0WVK2
-
-
Metarhizium brunneum Ma10 A0A6C0WW14
-
-
Metarhizium brunneum Ma10 A0A6C0WWH2
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Metarhizium brunneum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Metarhizium brunneum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitropropane + O2
-
 Metarhizium brunneum propionaldehyde + HNO2
-
 ?
1-nitropropane + O2
-
 Metarhizium brunneum Ma10 propionaldehyde + HNO2
-
 ?
2-nitropropane + O2
-
 Metarhizium brunneum acetone + HNO2
-
 ?
2-nitropropane + O2
-
 Metarhizium brunneum Ma10 acetone + HNO2
-
 ?
nitroethane + O2
-
 Metarhizium brunneum ?
-
 ?
nitroethane + O2
-
 Metarhizium brunneum Ma10 ?
-
 ?

Synonyms

Synonyms Comment Organism
NMO
-
 Metarhizium brunneum
Npd1
-
 Metarhizium brunneum
Npd2
-
 Metarhizium brunneum
Npd3
-
 Metarhizium brunneum
Npd4
-
 Metarhizium brunneum
Npd5
-
 Metarhizium brunneum
Npd6
-
 Metarhizium brunneum

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Metarhizium brunneum

Expression

Organism Comment Expression
Metarhizium brunneum the enzyme is expressed during Metarhizium brunneum infection of Plutella xylostella up