Literature summary for 1.13.12.13 extracted from

Gibbons, A.E.; Luker, K.E.; Luker, G.D.
Dual reporter bioluminescence imaging with nanoLuc and firefly luciferase (2018), Methods Mol. Biol., 1790, 41-50 .

Search for more literature for 1.13.12.13

Application

Application	Comment	Organism
molecular biology	bioluminescence imaging is a powerful, broadly utilized method for non-invasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. The use of a luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, is described in cell-based assays bioluminescence imaging of tumor-bearing mice. NanoLuc is combined with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session	Oplophorus gracilirostris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Oplophorus luciferin + O2	Oplophorus gracilirostris	-	oxidized Oplophorus luciferin + CO2 + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Oplophorus gracilirostris	Q9GV45 AND Q9GV46	Q9GV45: catalytic subunit, Q9GV46: non-catalytic subunit	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Oplophorus luciferin + O2	-	Oplophorus gracilirostris	oxidized Oplophorus luciferin + CO2 + hv	-	?

Synonyms

Synonyms	Comment	Organism
NanoLuc	-	Oplophorus gracilirostris

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Release 2023.1 (January 2023)