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Literature summary for 1.13.12.13 extracted from

  • Baba, Y.; Yamamoto, K.; Yoshida, W.
    Multicolor bioluminescence resonance energy transfer assay for quantification of global DNA methylation (2019), Anal. Bioanal. Chem., 411, 4765-4773 .
    View publication on PubMed

Application

Application Comment Organism
molecular biology a multicolor BRET assay for the quantification of global DNA methylation is developed using CXXC-fused Oplophorus luciferase (CXXC-Oluc), a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc), BOBO-1, and BOBO-3. CXXC-Oluc recognized unmethylated CpG sites on genomic DNA to excite BOBO-1 DNA intercalating dye, whereas MBD-Fluc recognized methylated CpG sites on genomic DNA to excite BOBO-3 DNA intercalating dye. The emission intensities of BOBO-1 and BOBO-3 are simultaneously detected and depended on the unmethylated and methylated CpG contents of the genomic DNA. There is a significant negative correlation between the emission intensities of BOBO-1 and BOBO-3. Therefore, the global DNA methylation level can be quantified with this multicolor BRET assay using a single tube Oplophorus sp.

Organism

Organism UniProt Comment Textmining
Oplophorus sp.
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Synonyms

Synonyms Comment Organism
CXXC-Oluc
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Oplophorus sp.
Oplophorus luciferase
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Oplophorus sp.