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Literature summary for 1.13.12.13 extracted from

  • Inouye, S.; Sato, J.; Sahara-Miura, Y.; Hosoya, T.; Suzuki, T.
    Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells (2014), Biochem. Biophys. Res. Commun., 450, 1313-1319 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates Oplophorus gracilirostris

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide Oplophorus gracilirostris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme possesses an amino-terminal signal peptide and is secreted Oplophorus gracilirostris
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
full-length enzyme Oplophorus gracilirostris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coelenterazine + O2 Oplophorus gracilirostris
-
coelenteramide + CO2 + hv
-
?
Oplophorus luciferin + O2 Oplophorus gracilirostris
-
oxidized Oplophorus luciferin + CO2 + hv
-
?

Organism

Organism UniProt Comment Textmining
Oplophorus gracilirostris Q9GV45 AND Q9GV46 catalytic and noncatalytic subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2
-
Oplophorus gracilirostris coelenteramide + CO2 + hv
-
?
Oplophorus luciferin + O2
-
Oplophorus gracilirostris oxidized Oplophorus luciferin + CO2 + hv
-
?

Subunits

Subunits Comment Organism
heterooligomer x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE Oplophorus gracilirostris

Synonyms

Synonyms Comment Organism
KAZ catalytic enzyme subunit Oplophorus gracilirostris
Oplophorus luciferase
-
Oplophorus gracilirostris

General Information

General Information Comment Organism
malfunction recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway Oplophorus gracilirostris