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Literature summary for 1.13.12.13 extracted from

  • Inouye, S.; Sahara-Miura, Y.; Sato, J.; Iimori, R.; Yoshida, S.; Hosoya, T.
    Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines (2013), Protein Expr. Purif., 88, 150-156.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C Oplophorus gracilirostris

Organism

Organism UniProt Comment Textmining
Oplophorus gracilirostris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using nickel-chelate affinity column Oplophorus gracilirostris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3iso-coelenterazine + O2 luminescence intesity (Imax): 78.2% Oplophorus gracilirostris oxidized 3iso-coelenterazine + CO2 + hn
-
?
3me-coelenterazine + O2 luminescence intesity (Imax): 80% Oplophorus gracilirostris oxidized 3me-coelenterazine + CO2 + hn
-
?
3meo-coelenterazine + O2 luminescence intesity (Imax): 189% Oplophorus gracilirostris oxidized 3meo-coelenterazine + CO2 + hn
-
?
alphameh-coelenterazine + O2 luminescence intesity (Imax): 15.7% Oplophorus gracilirostris oxidized alphameh-coelenterazine + CO2 + hn
-
?
cf3-coelenterazine + O2 luminescence intesity (Imax): 49.5% Oplophorus gracilirostris oxidized cf3-coelenterazine + CO2 + hn
-
?
coelenterazine + O2 luminescence intesity (Imax): 100% Oplophorus gracilirostris oxidized coelenterazine + CO2 + hn
-
?
et-coelenterazine + O2 luminescence intesity (Imax): 21.5% Oplophorus gracilirostris oxidized et-coelenterazine + CO2 + hn
-
?
h-coelenterazine + O2 luminescence intesity (Imax): 68.4% Oplophorus gracilirostris oxidized h-coelenterazine + CO2 + hn
-
?
i-coelenterazine + O2 luminescence intesity (Imax): 32.3% Oplophorus gracilirostris oxidized i-coelenterazine + CO2 + hn
-
?
me-coelenterazine + O2 luminescence intesity (Imax): 46.6% Oplophorus gracilirostris oxidized me-coelenterazine + CO2 + hn
-
?
meo-coelenterazine + O2 luminescence intesity (Imax): 68.1% Oplophorus gracilirostris oxidized meo-coelenterazine + CO2 + hn
-
?

Synonyms

Synonyms Comment Organism
19kOLase
-
Oplophorus gracilirostris
luciferase
-
Oplophorus gracilirostris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Oplophorus gracilirostris