Cloned (Comment) | Organism |
---|---|
the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C | Oplophorus gracilirostris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oplophorus gracilirostris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using nickel-chelate affinity column | Oplophorus gracilirostris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3iso-coelenterazine + O2 | luminescence intesity (Imax): 78.2% | Oplophorus gracilirostris | oxidized 3iso-coelenterazine + CO2 + hn | - |
? | |
3me-coelenterazine + O2 | luminescence intesity (Imax): 80% | Oplophorus gracilirostris | oxidized 3me-coelenterazine + CO2 + hn | - |
? | |
3meo-coelenterazine + O2 | luminescence intesity (Imax): 189% | Oplophorus gracilirostris | oxidized 3meo-coelenterazine + CO2 + hn | - |
? | |
alphameh-coelenterazine + O2 | luminescence intesity (Imax): 15.7% | Oplophorus gracilirostris | oxidized alphameh-coelenterazine + CO2 + hn | - |
? | |
cf3-coelenterazine + O2 | luminescence intesity (Imax): 49.5% | Oplophorus gracilirostris | oxidized cf3-coelenterazine + CO2 + hn | - |
? | |
coelenterazine + O2 | luminescence intesity (Imax): 100% | Oplophorus gracilirostris | oxidized coelenterazine + CO2 + hn | - |
? | |
et-coelenterazine + O2 | luminescence intesity (Imax): 21.5% | Oplophorus gracilirostris | oxidized et-coelenterazine + CO2 + hn | - |
? | |
h-coelenterazine + O2 | luminescence intesity (Imax): 68.4% | Oplophorus gracilirostris | oxidized h-coelenterazine + CO2 + hn | - |
? | |
i-coelenterazine + O2 | luminescence intesity (Imax): 32.3% | Oplophorus gracilirostris | oxidized i-coelenterazine + CO2 + hn | - |
? | |
me-coelenterazine + O2 | luminescence intesity (Imax): 46.6% | Oplophorus gracilirostris | oxidized me-coelenterazine + CO2 + hn | - |
? | |
meo-coelenterazine + O2 | luminescence intesity (Imax): 68.1% | Oplophorus gracilirostris | oxidized meo-coelenterazine + CO2 + hn | - |
? |
Synonyms | Comment | Organism |
---|---|---|
19kOLase | - |
Oplophorus gracilirostris |
luciferase | - |
Oplophorus gracilirostris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Oplophorus gracilirostris |