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Literature summary for 1.13.11.92 extracted from
- A dual function alpha-dioxygenase-peroxidase and NAD(+) oxidoreductase active enzyme from germinating pea rationalizing alpha-oxidation of fatty acids in plants (2000), Plant Physiol., 123, 1545-1552 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
lauric acid | pH 7.5, 30°C | Pisum sativum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230000 | - |
gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from germinating pea, using (NH4)2SO4 and pH precipitations and three successive chromatographic steps | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | germinating fruit | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.94 | - |
pH 7.5, 30°C | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lauric acid + O2 | - |
Pisum sativum | (R)-2-hydroperoxydodecanoic acid | - |
? | |
| additional information | enzyme shows both peroxidase and NAD+ oxidoreductase activity | Pisum sativum | ? | - |
- |
|
| palmitic acid + O2 | - |
Pisum sativum | (R,S)-2-hydroperoxypalmitic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 50000, plus x * 70000, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pisum sativum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | - |
Pisum sativum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | isoelectric focussing | - |
4.7 |
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Release 2023.1 (January 2023)
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