Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000154 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16585 | - |
4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
16716 | - |
4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
62300 | - |
sedimentation equilibrium analysis | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin + O2 | Mycobacterium tuberculosis | - |
7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNC5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin + O2 | - |
Mycobacterium tuberculosis | 7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
homotetramer | 4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DHNA | - |
Mycobacterium tuberculosis |
dihydroneopterin oxygenase | - |
Mycobacterium tuberculosis |
Rv3607c | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis |