Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudarthrobacter phenanthrenivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Pseudarthrobacter phenanthrenivorans | |
Mn2+ | strong inhibition | Pseudarthrobacter phenanthrenivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
3,4-dihydroxybenzoate | pH 9.5, 20°C, recombinant enzyme | Pseudarthrobacter phenanthrenivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | more than a 6-fold increase in specific activity after incubation of the enzyme with 0.2 mM Fe(II) and L-ascorbic acid | Pseudarthrobacter phenanthrenivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | Pseudarthrobacter phenanthrenivorans | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
3,4-dihydroxybenzoate + O2 | Pseudarthrobacter phenanthrenivorans Sphe3 | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 1% (v/v), stabilizes | Pseudarthrobacter phenanthrenivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudarthrobacter phenanthrenivorans | F0MCP7 | - |
- |
Pseudarthrobacter phenanthrenivorans Sphe3 | F0MCP7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudarthrobacter phenanthrenivorans |
Storage Stability | Organism |
---|---|
-80 °C, in the presence of 10% (v/v) glycerol, purified enzyme maintains 93% of its activity for up to at least 4 months | Pseudarthrobacter phenanthrenivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudarthrobacter phenanthrenivorans | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Pseudarthrobacter phenanthrenivorans Sphe3 | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
Synonyms | Comment | Organism |
---|---|---|
PcaA | - |
Pseudarthrobacter phenanthrenivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Pseudarthrobacter phenanthrenivorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | 10°C: about 20% of maximal activity, 45°C: about 20% of maximal activity | Pseudarthrobacter phenanthrenivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudarthrobacter phenanthrenivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | active within pH 5.5 and 10.5, no activity at pH 5.0, 1nd 11.0 | Pseudarthrobacter phenanthrenivorans |
Organism | Comment | Expression |
---|---|---|
Pseudarthrobacter phenanthrenivorans | the expression of pcaA gene is induced in cells grown on phenanthrene or 3,4-dihydroxybenzoate showing up to 139- and 304-fold change increase in mRNA expression levels in the presence of phenanthrene and 3,4-dihydroxybenzoate, respectively, when compared to glucose | up |