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Literature summary for 1.13.11.79 extracted from

  • Collins, H.F.; Biedendieck, R.; Leech, H.K.; Gray, M.; Escalante-Semerena, J.C.; McLean, K.J.; Munro, A.W.; Rigby, S.E.; Warren, M.J.; Lawrence, A.D.
    Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species (2013), PLoS ONE, 8, e55708.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Salmonella enterica strain JE9385 and in Escherichia coli Priestia megaterium
expressed in Salmonella enterica strain JE9385 and in Escherichia coli Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Rhodobacter capsulatus
27300
-
x * 27300, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMNH2 + O2 Priestia megaterium
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + urea + ?
-
?
FMNH2 + O2 Rhodobacter capsulatus
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + urea + ?
-
?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity chromatography Priestia megaterium
metal affinity chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMNH2 + O2
-
Priestia megaterium 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + urea + ?
-
?
FMNH2 + O2
-
Rhodobacter capsulatus 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + urea + ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Rhodobacter capsulatus
? x * 27300, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
BluB
-
Priestia megaterium
BluB
-
Rhodobacter capsulatus
DMB-synthase
-
Priestia megaterium
DMB-synthase
-
Rhodobacter capsulatus