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Literature summary for 1.13.11.74 extracted from

  • Lendenmann, U.; Spain, J.C.
    2-Aminophenol 1,6-dioxygenase: a novel aromatic ring cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45 (1996), J. Bacteriol., 178, 6227-6232.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
dialysis against 50 mM MOPS (pH 7.3) containing ethanol (10% [v/v]) for 24 h abolishes 95% of the activity of 2-aminophenol 1,6-dioxygenase Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
1,2,3-trihydroxybenzene complete inhibition at 0.05 mM Pseudomonas oleovorans
1,2,4-Trihydroxybenzene complete inhibition at 0.05 mM Pseudomonas oleovorans
2,2'-dipyridyl complete inhibition at 2 mM Pseudomonas oleovorans
2,3-Dihydroxybenzoic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
2-amino-p-cresol 90% inhibition at 0.05 mM Pseudomonas oleovorans
2-methoxyphenol 30% inhibition at 0.05 mM Pseudomonas oleovorans
3-Amino-4-hydroxybenzoic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
3-chlorocatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
3-hydroxyanthranilic acid 20% inhibition at 0.05 mM Pseudomonas oleovorans
3-methylcatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
4-amino-3-hydroxybenzoic acid 30% inhibition at 0.05 mM Pseudomonas oleovorans
4-amino-m-cresol 10% inhibition at 0.05 mM Pseudomonas oleovorans
4-aminoresorcinol complete inhibition at 0.05 mM Pseudomonas oleovorans
4-Chlorocatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
4-methylcatechol complete inhibition at 0.05 mM Pseudomonas oleovorans
Ca2+ 84% residual activity at 2 mM Pseudomonas oleovorans
Cd2+ complete inhibition at 2 mM Pseudomonas oleovorans
Co2+ 47% residual activity at 2 mM Pseudomonas oleovorans
EDTA 70% residual activity at 2 mM Pseudomonas oleovorans
Fe3+ 78% residual activity at 2 mM Pseudomonas oleovorans
H2O2 complete inhibition at 1 mM Pseudomonas oleovorans
K3Fe(CN)6 17% residual activity at 1 mM Pseudomonas oleovorans
Mg2+ 85% residual activity at 2 mM Pseudomonas oleovorans
Mn2+ 5% residual activity at 2 mM Pseudomonas oleovorans
Mo2+ 96% residual activity at 2 mM Pseudomonas oleovorans
Ni2+ 50% residual activity at 2 mM Pseudomonas oleovorans
nitrilotriacetic acid 86% residual activity at 2 mM Pseudomonas oleovorans
o-phenanthroline 1% residual activity at 2 mM Pseudomonas oleovorans
protocatechuate 20% inhibition at 0.05 mM Pseudomonas oleovorans
Zn2+ complete inhibition at 2 mM Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
2-Aminophenol in 50 mM Tris (pH 8.0) at 25┬░C Pseudomonas oleovorans
0.71
-
O2 in 50 mM Tris (pH 8.0) at 25┬░C Pseudomonas oleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ferrous iron is the sole cofactor, 100% activity at 2 mM Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans
39000
-
2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans
140000
-
gel filtration Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-
Pseudomonas oleovorans JS45
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ethanol precipitation, MonoQ column chromatography, and Sephacryl S-300 gel filtration Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.5
-
crude extract, in 50 mM Tris (pH 8.0) at 25┬░C Pseudomonas oleovorans
21.6
-
purified enzyme, in 50 mM Tris (pH 8.0) at 25┬░C Pseudomonas oleovorans

Storage Stability

Storage Stability Organism
-20┬░C, purified enzyme in 50 mM MOPS at pH 7.3 in the presence of 10% (v/v) glycerol, several months, no loss of activity Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-chlorophenol + O2 15% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
?
2-amino-4-chlorophenol + O2 15% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
?
2-amino-m-cresol + O2 1% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
?
2-amino-m-cresol + O2 1% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
?
2-aminophenol + O2 100% activity Pseudomonas oleovorans 2-aminomuconate 6-semialdehyde
-
?
2-aminophenol + O2 100% activity Pseudomonas oleovorans JS45 2-aminomuconate 6-semialdehyde
-
?
6-amino-m-cresol + O2 25% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
?
catechol + O2 13% activity compared to 2-aminophenol Pseudomonas oleovorans ?
-
?
catechol + O2 13% activity compared to 2-aminophenol Pseudomonas oleovorans JS45 ?
-
?
additional information 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates Pseudomonas oleovorans ?
-
?
additional information 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, 1,2,3-trihydroxybenzene, 1,2,4-trihydroxybenzene, 4-aminoresorcinol, 2-amino-p-cresol, 2,3-dihydroxybenzoic acid, protocatechuate, 2-methoxyphenol, 4-amino-3-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid, 3-hydroxyanthranilic acid, and 4-amino-m-cresol are no substrates Pseudomonas oleovorans JS45 ?
-
?

Subunits

Subunits Comment Organism
heterodimer 2 * 39000 + 2 * 35000, SDS-PAGE Pseudomonas oleovorans

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
the enzyme loses 50% of its activity upon incubation at 60┬░C for 5 min Pseudomonas oleovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
147
-
2-Aminophenol in 50 mM Tris (pH 8.0) at 25┬░C Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Pseudomonas oleovorans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5
-
Pseudomonas oleovorans