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Literature summary for 1.13.11.74 extracted from

  • Wu, J.F.; Sun, C.W.; Jiang, C.Y.; Liu, Z.P.; Liu, S.J.
    A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli (2005), Arch. Microbiol., 183, 1-8.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 79.4% residual activity at 2 mM Comamonas testosteroni
Cd2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
Co2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
Cu2+ 2.9% residual activity at 2 mM Comamonas testosteroni
EDTA
-
Comamonas testosteroni
Fe2+ 57.4% residual activity at 2 mM Comamonas testosteroni
Fe3+ 4.4% residual activity at 2 mM Comamonas testosteroni
H2O2 abolishes enzyme activity completely at 2 mM Comamonas testosteroni
K3Fe(CN)6 41.2% residual activity at 2 mM Comamonas testosteroni
Mg2+ 91.2% residual activity at 2 mM Comamonas testosteroni
Mn2+ 30.9% residual activity at 2 mM Comamonas testosteroni
Ni2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
Zn2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00077
-
2-amino-5-chlorophenol in 10 mM sodium phosphate buffer, at pH 8.0 and 22┬░C Comamonas testosteroni
0.00089
-
2-Aminophenol in 10 mM sodium phosphate buffer, at pH 8.0 and 22┬░C Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni
38000
-
2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni
130000
-
gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-5-chlorophenol + O2 Comamonas testosteroni 68% activity compared to 2-aminophenol ?
-
?
2-amino-5-chlorophenol + O2 Comamonas testosteroni CNB-1 68% activity compared to 2-aminophenol ?
-
?
2-aminophenol + O2 Comamonas testosteroni 100% activity 2-aminomuconate 6-semialdehyde
-
?
2-aminophenol + O2 Comamonas testosteroni CNB-1 100% activity 2-aminomuconate 6-semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-
Comamonas testosteroni CNB-1 Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5-chlorophenol + O2 68% activity compared to 2-aminophenol Comamonas testosteroni ?
-
?
2-amino-5-chlorophenol + O2 68% activity compared to 2-aminophenol Comamonas testosteroni CNB-1 ?
-
?
2-aminophenol + O2 100% activity Comamonas testosteroni 2-aminomuconate 6-semialdehyde
-
?
2-aminophenol + O2 100% activity Comamonas testosteroni CNB-1 2-aminomuconate 6-semialdehyde
-
?
additional information 5% activity with catechol Comamonas testosteroni ?
-
?
additional information 5% activity with catechol Comamonas testosteroni CNB-1 ?
-
?
protocatechuate + O2 33% activity compared to 2-aminophenol Comamonas testosteroni ?
-
?
protocatechuate + O2 33% activity compared to 2-aminophenol Comamonas testosteroni CNB-1 ?
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2
-
in 10 mM sodium phosphate buffer, at pH 8.0 and 22┬░C Comamonas testosteroni EDTA