Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Comamonas testosteroni |
Crystallization (Comment) | Organism |
---|---|
in complex with (4Z,6Z)-3-iminooxepin-2(3H)-one, 2-aminomuconic 6-semialdehyde and 4-nitrocatechol, hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 mM sodium chloride, 0.1 M sodium cacodylate pH 6.5 | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
E251A | inactive | Comamonas testosteroni |
H13A | inactive | Comamonas testosteroni |
H195Q | inactive | Comamonas testosteroni |
H62A | inactive | Comamonas testosteroni |
Y129F | inactive | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrocatechol | suicide inhibitor, the enzyme completely loses its activity towards 2-aminophenol after incubation with 4-nitrocatechol for 100 s | Comamonas testosteroni | |
catechol | the enzyme completely loses its activity towards 2-aminophenol after incubation with catechol for 100 s | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0171 | - |
2-Aminophenol | at pH 8.0, temperature not specified in the publication | Comamonas testosteroni | |
0.0775 | - |
O2 | at pH 8.0, temperature not specified in the publication | Comamonas testosteroni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains Fe2+ | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminophenol + O2 | Comamonas testosteroni | - |
2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | Comamonas testosteroni CNB-1 | - |
2-aminomuconate 6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Comamonas testosteroni CNB-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminophenol + O2 | - |
Comamonas testosteroni | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | - |
Comamonas testosteroni CNB-1 | 2-aminomuconate 6-semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
APD | - |
Comamonas testosteroni |