BRENDA - Enzyme Database show
show all sequences of 1.13.11.71

Ablation of beta,beta-carotene-9,10-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice

Guo, X.; Wu, L.; Lyu, Y.; Chowanadisai, W.; Clarke, S.L.; Lucas, E.A.; Smith, B.J.; He, H.; Wang, W.; Medeiros, D.M.; Lin, D.; J. Nutr. Biochem. 46, 74-82 (2017)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Mus musculus
5743
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-beta-carotene + O2
Mus musculus
-
all-trans-10'-apo-beta-carotenal + beta-ionone
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q99NF1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-beta-carotene + O2
-
745596
Mus musculus
all-trans-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
-
Mus musculus
5743
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-beta-carotene + O2
Mus musculus
-
all-trans-10'-apo-beta-carotenal + beta-ionone
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-beta-carotene + O2
-
745596
Mus musculus
all-trans-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
systemic depletion of BCO2 causes increased food intake and impaired hepatic lipid metabolism in mice. Compared to wild-type, BCO2 knockout mice exhibit widespread disruptions in metabolism and metabolite homeostasis, an increase in fasting blood glucose, a decrease in circulating glucagon and leptin, an elevation of plasma interleukin 1 beta and tumor necrosis factor alpha, and impaired AMP activated protein kinase signaling. The global hypothalamic metabolomic results reveal that depletion of BCO2 results in striking metabolic changes, including suppression of long-chain fatty acids transport into mitochondria, inhibition of the metabolism of dipeptides and sulfur-containing amino acids, and stimulation of local oxidative stress and inflammation in the hypothalamus of BCO2 knockout mice. Complex interplay between the hormone signaling and impaired lipid and glucose metabolism seems to account for initiation of oxidative stress, inflammation and eventual metabolic disorders in BCO2 knockout mice. Phenotype, overview
Mus musculus
physiological function
the enzyme catalyzes the asymmetric cleavage of carotenoids. Enzyme BCO2 exerts its role in hypothalamic nutrient metabolism and feeding behavior. BCO2 regulates hypothalamic mitochondrial function, nutrient metabolism, and local oxidative stress and inflammation
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
systemic depletion of BCO2 causes increased food intake and impaired hepatic lipid metabolism in mice. Compared to wild-type, BCO2 knockout mice exhibit widespread disruptions in metabolism and metabolite homeostasis, an increase in fasting blood glucose, a decrease in circulating glucagon and leptin, an elevation of plasma interleukin 1 beta and tumor necrosis factor alpha, and impaired AMP activated protein kinase signaling. The global hypothalamic metabolomic results reveal that depletion of BCO2 results in striking metabolic changes, including suppression of long-chain fatty acids transport into mitochondria, inhibition of the metabolism of dipeptides and sulfur-containing amino acids, and stimulation of local oxidative stress and inflammation in the hypothalamus of BCO2 knockout mice. Complex interplay between the hormone signaling and impaired lipid and glucose metabolism seems to account for initiation of oxidative stress, inflammation and eventual metabolic disorders in BCO2 knockout mice. Phenotype, overview
Mus musculus
physiological function
the enzyme catalyzes the asymmetric cleavage of carotenoids. Enzyme BCO2 exerts its role in hypothalamic nutrient metabolism and feeding behavior. BCO2 regulates hypothalamic mitochondrial function, nutrient metabolism, and local oxidative stress and inflammation
Mus musculus
Other publictions for EC 1.13.11.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745596
Guo
Ablation of beta,beta-caroten ...
Mus musculus
J. Nutr. Biochem.
46
74-82
2017
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2
2
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745345
Dela Sena
Substrate specificity of puri ...
Gallus gallus, Homo sapiens
J. Biol. Chem.
291
14609-14619
2016
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2
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1
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2
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11
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2
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2
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3
3
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745753
Gong
Mitochondrial beta-carotene 9 ...
Homo sapiens
Mol. Cancer Res.
14
966-975
2016
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8
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1
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2
3
3
2
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744160
Kim
Tissue- and sex-specific effe ...
Mus musculus, Mus musculus C57BL/6
Arch. Biochem. Biophys.
572
11-18
2015
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2
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2
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744161
Raghuvanshi
Cellular localization of ?-ca ...
Rattus norvegicus
Arch. Biochem. Biophys.
572
19-27
2015
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2
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1
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1
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1
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1
1
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744574
Wei
Purification and characteriza ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ULI3
Biotechnol. Lett.
37
1993-1998
2015
3
1
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5
3
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5
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8
8
4
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1
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1
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10
1
1
1
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1
1
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1
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5
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5
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8
8
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1
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1
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10
1
1
1
-
3
1
1
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1
1
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3
3
744613
Jlali
Nutrigenetics of carotenoid m ...
Gallus gallus
Br. J. Nutr.
111
2079-2088
2014
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1
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1
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1
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3
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746290
Li
Inactivity of human beta,beta ...
Homo sapiens, Mus musculus, Mus musculus C57/BL6
Proc. Natl. Acad. Sci. USA
111
10173-10178
2014
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2
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2
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10
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10
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2
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11
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2
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10
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2
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11
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2
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2
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5
5
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727965
Amengual
Two carotenoid-oxygenases cont ...
Mus musculus
J. Biol. Chem.
288
34081-34096
2013
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1
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1
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1
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1
1
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728057
Liu
Carotenoid cleavage dioxygenas ...
Lotus japonicus
J. Exp. Bot.
64
1967-1981
2013
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1
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1
1
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727353
Lobo
BCDO2 acts as a carotenoid sca ...
Danio rerio, Homo sapiens
Development
139
2966-2977
2012
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3
3
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719151
Kim
Production of beta-apo-10'-car ...
Homo sapiens
Biotechnol. Lett.
33
1195-1200
2011
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1
1
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1
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1
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1
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5
1
1
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1
1
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1
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1
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1
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1
-
5
1
1
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1
1
719438
Amengual
A mitochondrial enzyme degrade ...
Mus musculus
FASEB J.
25
948-959
2011
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1
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1
1
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728552
Amengual
Beta-carotene reduces body adi ...
Mus musculus
PLoS ONE
6
e20644
2011
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1
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714616
Vage
A nonsense mutation in the bet ...
Ovis aries
BMC Genet.
11
10
2010
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1
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1
1
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720559
Ledger
Modified CAROTENOID CLEAVAGE D ...
Actinidia chinensis
New Phytol.
188
803-813
2010
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1
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720702
Auldridge
Characterization of three memb ...
Arabidopsis thaliana
Plant J.
45
982-993
2006
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1
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720152
Lindqvist
Cell type-specific expression ...
Homo sapiens
J. Histochem. Cytochem.
53
1403-1412
2005
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719823
Schwartz
The biochemical characterizati ...
Arabidopsis thaliana
J. Biol. Chem.
279
46940-46945
2004
1
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1
1
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719806
Kiefer
Identification and characteriz ...
Homo sapiens, Mus musculus
J. Biol. Chem.
276
14110-14116
2001
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