Any feedback?
Literature summary for 1.13.11.71 extracted from
- Purification and characterization of a novel beta-carotene-9',10'-oxygenase from Saccharomyces cerevisiae ULI3 (2015), Biotechnol. Lett., 37, 1993-1998 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | activates by 27% at 5 mM | Saccharomyces cerevisiae |  |
| Triton X-100 | activates by 7% at 1% w/v | Saccharomyces cerevisiae | |
| Tween 20 | activates by 3% at 1% w/v | Saccharomyces cerevisiae | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme ScBCO2 potentially can be used for the enzymatic biotransformation of beta-carotene to beta-apo-10'-carotenal in biotechnological applications | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibits by 10% at 5 mM | Saccharomyces cerevisiae | |
| Fe2+ | inhibits by 37% at 5 mM | Saccharomyces cerevisiae | |
| Mn2+ | inhibits by 13% at 5 mM | Saccharomyces cerevisiae | |
| SDS | 99% inhibition at 1 % | Saccharomyces cerevisiae | |
| Urea | 86% inhibition at 4 M | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 0.048 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 0.105 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates by 26% at 5 mM | Saccharomyces cerevisiae | |
| Cu2+ | activates by 23% at 5 mM | Saccharomyces cerevisiae | |
| Mg2+ | activates by 14% at 5 mM | Saccharomyces cerevisiae | |
| Ni2+ | activates by 7% at 5 mM | Saccharomyces cerevisiae | |
| Zn2+ | activates by 17% at 5 mM | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-beta-carotene + O2 | Saccharomyces cerevisiae | - |
all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| all-trans-beta-carotene + O2 | Saccharomyces cerevisiae ULI3 | - |
all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| alpha-carotene + O2 | Saccharomyces cerevisiae | - |
beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
| alpha-carotene + O2 | Saccharomyces cerevisiae ULI3 | - |
beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
| lutein + O2 | Saccharomyces cerevisiae | - |
? | - |
? | |
| lutein + O2 | Saccharomyces cerevisiae ULI3 | - |
? | - |
? | |
| additional information | Saccharomyces cerevisiae | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | ? | - |
? | |
| additional information | Saccharomyces cerevisiae ULI3 | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-butanone | inhibits 66% at 50 v/v | Saccharomyces cerevisiae |
| Acetone | inhibits 20% at 50 v/v | Saccharomyces cerevisiae |
| benzene | inhibits 28% at 50 v/v | Saccharomyces cerevisiae |
| chloroform | inhibits 57% at 50 v/v | Saccharomyces cerevisiae |
| dichloromethane | inhibits 49% at 50 v/v | Saccharomyces cerevisiae |
| dimethylformamide | inhibits 24% at 50 v/v | Saccharomyces cerevisiae |
| DMSO | inhibits 17% at 50 v/v | Saccharomyces cerevisiae |
| toluene | inhibits 28% at 50 v/v | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae ULI3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 9.5fold to homogeneity by ammonium sulfate fractionation and gel filtration | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.38 | - |
purified native enzyme, pH 8.0, 45°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-beta-carotene + O2 | - |
Saccharomyces cerevisiae | all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| all-trans-beta-carotene + O2 | - |
Saccharomyces cerevisiae ULI3 | all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
| alpha-carotene + O2 | - |
Saccharomyces cerevisiae | beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
| alpha-carotene + O2 | - |
Saccharomyces cerevisiae ULI3 | beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
| lutein + O2 | - |
Saccharomyces cerevisiae | ? | - |
? | |
| lutein + O2 | - |
Saccharomyces cerevisiae ULI3 | ? | - |
? | |
| additional information | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | enzyme ScBCO2 only exhibits cleavage activity towards carotenoid substrates containing two beta-ionone rings and its catalytic efficiency (kcat/Km) follows the descending order beta-carotene, alpha-carotene, lutein | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | Saccharomyces cerevisiae ULI3 | ? | - |
? | |
| additional information | enzyme ScBCO2 only exhibits cleavage activity towards carotenoid substrates containing two beta-ionone rings and its catalytic efficiency (kcat/Km) follows the descending order beta-carotene, alpha-carotene, lutein | Saccharomyces cerevisiae ULI3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-carotene-9,10'-oxygenase | - |
Saccharomyces cerevisiae |
| ScBCO2 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | activity range, profile overview | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0167 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 0.0392 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 0.0475 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 50 % of the maximal activity at pH 7.0 and pH 9.0, 20% at pH 6.0 and pH 9.5 | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme converts beta-carotene to beta-apo-10'-carotenal, which is a precursor of the plant hormone strigolactone | Saccharomyces cerevisiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.159 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 0.817 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
| 1.284 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
