BRENDA - Enzyme Database show
show all sequences of 1.13.11.71

The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching

Schwartz, S.H.; Qin, X.; Loewen, M.C.; J. Biol. Chem. 279, 46940-46945 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
methanol
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0152
-
beta-carotene
pH 7.0, temperature not specified in the publication
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
protein contains a probable chloroplast targeting sequence of 31 amino acids
Arabidopsis thaliana
9507
-
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Methanol
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q7XJM2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-carotene + O2
-
719823
Arabidopsis thaliana
10'-apo-beta-carotenal + beta-ionone
-
-
-
?
additional information
no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin
719823
Arabidopsis thaliana
?
-
-
-
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
methanol
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a truncated enzyme protein of 587 amino acids lacking the N-terminal targeting sequence is also able to catalyze the 910 cleavage of beta-carotene. Coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0152
-
beta-carotene
pH 7.0, temperature not specified in the publication
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
protein contains a probable chloroplast targeting sequence of 31 amino acids
Arabidopsis thaliana
9507
-
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Methanol
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-carotene + O2
-
719823
Arabidopsis thaliana
10'-apo-beta-carotenal + beta-ionone
-
-
-
?
additional information
no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin
719823
Arabidopsis thaliana
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching
Arabidopsis thaliana
Other publictions for EC 1.13.11.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745596
Guo
Ablation of beta,beta-caroten ...
Mus musculus
J. Nutr. Biochem.
46
74-82
2017
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2
2
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745345
Dela Sena
Substrate specificity of puri ...
Gallus gallus, Homo sapiens
J. Biol. Chem.
291
14609-14619
2016
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2
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11
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2
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2
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3
3
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745753
Gong
Mitochondrial beta-carotene 9 ...
Homo sapiens
Mol. Cancer Res.
14
966-975
2016
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1
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2
3
3
2
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744160
Kim
Tissue- and sex-specific effe ...
Mus musculus, Mus musculus C57BL/6
Arch. Biochem. Biophys.
572
11-18
2015
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2
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744161
Raghuvanshi
Cellular localization of ?-ca ...
Rattus norvegicus
Arch. Biochem. Biophys.
572
19-27
2015
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2
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1
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4
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1
1
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744574
Wei
Purification and characteriza ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ULI3
Biotechnol. Lett.
37
1993-1998
2015
3
1
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5
3
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5
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8
8
4
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1
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10
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1
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3
1
1
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1
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5
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8
8
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1
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1
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10
1
1
1
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3
1
1
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1
1
-
3
3
744613
Jlali
Nutrigenetics of carotenoid m ...
Gallus gallus
Br. J. Nutr.
111
2079-2088
2014
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-
1
-
1
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1
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746290
Li
Inactivity of human beta,beta ...
Homo sapiens, Mus musculus, Mus musculus C57/BL6
Proc. Natl. Acad. Sci. USA
111
10173-10178
2014
-
-
2
-
2
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10
-
10
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2
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11
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2
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10
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2
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11
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2
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2
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5
5
-
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727965
Amengual
Two carotenoid-oxygenases cont ...
Mus musculus
J. Biol. Chem.
288
34081-34096
2013
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1
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1
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1
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1
1
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728057
Liu
Carotenoid cleavage dioxygenas ...
Lotus japonicus
J. Exp. Bot.
64
1967-1981
2013
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1
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1
1
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727353
Lobo
BCDO2 acts as a carotenoid sca ...
Danio rerio, Homo sapiens
Development
139
2966-2977
2012
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1
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3
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6
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3
3
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719151
Kim
Production of beta-apo-10'-car ...
Homo sapiens
Biotechnol. Lett.
33
1195-1200
2011
-
1
1
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1
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1
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1
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1
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1
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5
1
1
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1
1
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1
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1
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1
-
1
-
5
1
1
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-
1
1
719438
Amengual
A mitochondrial enzyme degrade ...
Mus musculus
FASEB J.
25
948-959
2011
-
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1
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1
1
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728552
Amengual
Beta-carotene reduces body adi ...
Mus musculus
PLoS ONE
6
e20644
2011
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714616
Vage
A nonsense mutation in the bet ...
Ovis aries
BMC Genet.
11
10
2010
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720559
Ledger
Modified CAROTENOID CLEAVAGE D ...
Actinidia chinensis
New Phytol.
188
803-813
2010
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720702
Auldridge
Characterization of three memb ...
Arabidopsis thaliana
Plant J.
45
982-993
2006
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1
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720152
Lindqvist
Cell type-specific expression ...
Homo sapiens
J. Histochem. Cytochem.
53
1403-1412
2005
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719823
Schwartz
The biochemical characterizati ...
Arabidopsis thaliana
J. Biol. Chem.
279
46940-46945
2004
1
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1
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719806
Kiefer
Identification and characteriz ...
Homo sapiens, Mus musculus
J. Biol. Chem.
276
14110-14116
2001
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