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Literature summary for 1.13.11.71 extracted from
- Production of beta-apo-10'-carotenal from beta-carotene by human beta-carotene-9',10'-oxygenase expressed in E. coli (2011), Biotechnol. Lett., 33, 1195-1200.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of beta-apo-10'-carotenal by recombinant enzyme. The optimum pH, temperature, detergent type, and the optimum concentrations of detergent, substrate, and enzyme for beta-apo-10'-carotenal production are 8.0, 37°C, Tween 40, 2.4%, 300 mg beta-carotene/l, and 0.25 U/ml, respectively, giving 43 mg beta-apo-10'-carotenal/l after 21 h with a conversion of 14% | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
beta-carotene | pH 8.0, 37°C | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BYV7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-carotene + O2 | - |
Homo sapiens | 10'-apo-carotenal + beta-ionone | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BCO2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
half-life 326 min | Homo sapiens |
| 40 | - |
half-life 247 min | Homo sapiens |
| 45 | - |
half-life 191 min | Homo sapiens |
| 50 | - |
half-life 84 min | Homo sapiens |
| 55 | - |
half-life 22 min | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0053 | - |
beta-carotene | pH 8.0, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
beta-carotene | pH 8.0, 37°C | Homo sapiens | |
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