BRENDA - Enzyme Database
show all sequences of 1.13.11.66

Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis

Miyauchi, K.; Adachi, Y.; Nagata, Y.; Takagi, M.; J. Bacteriol. 181, 6712-6719 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Sphingomonas paucimobilis
Engineering
Protein Variants
Commentary
Organism
E278A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
H162A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
H229A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
sequence contains a putative Fe(II)-binding site
Sphingomonas paucimobilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Sphingomonas paucimobilis
Organism
Organism
UniProt
Commentary
Textmining
Sphingomonas paucimobilis
Q9WXE6
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
crude extract, substrate hydroquinone, pH 7.0, 37C
Sphingomonas paucimobilis
0.88
-
crude extract, substrate 2-chlorohydroquinone, pH 7.0, 37C
Sphingomonas paucimobilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-chlorohydroquinone + O2
-
719693
Sphingomonas paucimobilis
?
ring cleavage product is an acylchloride, which reacts with water to give maleylacetate
-
-
?
hydroquinone + O2
-
719693
Sphingomonas paucimobilis
4-hydroxymuconic acid semialdehyde
-
-
-
?
additional information
enzyme cleaves aromatic rings with two hydroxyl groups ar para positions preferably. No substrate: catechol
719693
Sphingomonas paucimobilis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Sphingomonas paucimobilis
Synonyms
Synonyms
Commentary
Organism
LinE
-
Sphingomonas paucimobilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Sphingomonas paucimobilis
Engineering (protein specific)
Protein Variants
Commentary
Organism
E278A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
H162A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
H229A
mutation in putative Fe(II)-binding site, complete loss of activity
Sphingomonas paucimobilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
sequence contains a putative Fe(II)-binding site
Sphingomonas paucimobilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Sphingomonas paucimobilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
crude extract, substrate hydroquinone, pH 7.0, 37C
Sphingomonas paucimobilis
0.88
-
crude extract, substrate 2-chlorohydroquinone, pH 7.0, 37C
Sphingomonas paucimobilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-chlorohydroquinone + O2
-
719693
Sphingomonas paucimobilis
?
ring cleavage product is an acylchloride, which reacts with water to give maleylacetate
-
-
?
hydroquinone + O2
-
719693
Sphingomonas paucimobilis
4-hydroxymuconic acid semialdehyde
-
-
-
?
additional information
enzyme cleaves aromatic rings with two hydroxyl groups ar para positions preferably. No substrate: catechol
719693
Sphingomonas paucimobilis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Sphingomonas paucimobilis
Other publictions for EC 1.13.11.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744430
Ferraroni
The crystal structures of nat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Biochim. Biophys. Acta
1865
520-530
2017
-
-
-
1
-
-
2
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
6
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
745331
Liu
Crystal structure of PnpCD, a ...
Pseudomonas sp.
J. Biol. Chem.
290
24547-24560
2015
-
-
1
1
9
-
-
-
-
3
-
2
-
1
-
-
1
-
-
-
-
-
2
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
9
-
-
-
-
-
-
3
-
2
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
745802
Mancini
A copper-induced quinone degr ...
Lactococcus lactis, Lactococcus lactis IL1403
Mol. Microbiol.
95
645-659
2015
-
-
1
-
-
1
1
1
-
2
-
6
-
13
-
-
1
-
-
-
-
-
11
1
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
2
-
6
-
-
-
1
-
-
-
-
11
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp.
BMC Microbiol.
12
27
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
2
-
2
1
1
1
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
1
1
1
-
1
1
2
-
-
-
-
-
-
-
718555
Kolvenbach
Purification and characterizat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
AMB Express
1
08
2011
-
-
-
-
-
-
9
2
-
1
6
-
-
9
-
-
2
-
-
-
2
-
32
2
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
2
-
1
6
-
-
-
-
2
-
-
2
-
32
2
-
-
-
2
-
2
-
-
-
-
-
-
2
2
718916
Machonkin
Substrate specificity of Sphin ...
Sphingobium chlorophenolicum
Biochemistry
50
8899-8913
2011
-
-
-
-
-
-
3
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
12
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
719104
Shen
Cloning and characterization o ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Biores. Technol.
101
7516-7522
2010
-
-
-
-
2
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719327
Yin
Characterization of MnpC, a hy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Curr. Microbiol.
61
471-476
2010
-
-
1
-
-
-
-
1
-
2
1
-
-
28
-
-
-
-
-
-
1
3
4
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
3
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
720066
Machonkin
Determination of the active si ...
Sphingobium chlorophenolicum
J. Biol. Inorg. Chem.
15
291-301
2010
-
-
1
1
5
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719714
Moonen
Hydroquinone dioxygenase from ...
Pseudomonas fluorescens, Pseudomonas fluorescens ACB
J. Bacteriol.
190
5199-5209
2008
1
-
-
-
-
-
12
1
-
1
3
-
-
13
-
-
1
-
-
-
1
-
14
1
-
-
-
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
12
1
1
-
1
3
-
-
-
-
1
-
-
1
-
14
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
718727
Xun
Characterization of 2,6-dichlo ...
Sphingobium chlorophenolicum
Biochem. Biophys. Res. Commun.
266
322-325
1999
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
718831
Xu
Evidence that pcpA encodes 2,6 ...
Sphingobium chlorophenolicum
Biochemistry
38
7659-7669
1999
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719693
Miyauchi
Cloning and sequencing of a no ...
Sphingomonas paucimobilis
J. Bacteriol.
181
6712-6719
1999
-
-
1
-
3
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
2
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-