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Literature summary for 1.13.11.66 extracted from
- Characterization of MnpC, a hydroquinone dioxygenase likely involved in the meta-nitrophenol degradation by Cupriavidus necator JMP134 (2010), Curr. Microbiol., 61, 471-476.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cupriavidus necator |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.255 | - |
hydroquinone | pH 7.4, temperature not specified in the publication | Cupriavidus necator |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | preincubation in presence of 0.1 mM Fe2+ and 1 mM Mn2+ increases activity by 10fold | Cupriavidus necator | |
| Manganese | preincubation in presence of 0.1 mM Fe2+ and 1 mM Mn2+ increases activity by 10fold | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
- |
- |
| Cupriavidus necator JMP 134-1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.1 | - |
pH 7.4, temperature not specified in the publication | Cupriavidus necator |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 48% residual activity after 30 days | Cupriavidus necator |
| -70°C, 50% glycerol, 77% residual activity after 30 days | Cupriavidus necator |
| fairly stable for several hours on ice and retains approximately 80% of activity after 72 h | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroquinone + O2 | - |
Cupriavidus necator | 4-hydroxymuconic semialdehyde | - |
? | |
| hydroquinone + O2 | - |
Cupriavidus necator JMP 134-1 | 4-hydroxymuconic semialdehyde | - |
? | |
| additional information | no substrate: hydroxyquinol, catechol, 2-aminophenol,4-aminophenol, protocatechuate, and gentisate | Cupriavidus necator | ? | - |
? | |
| additional information | no substrate: hydroxyquinol, catechol, 2-aminophenol,4-aminophenol, protocatechuate, and gentisate | Cupriavidus necator JMP 134-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mnpC | gen name | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | - |
Cupriavidus necator |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
20% of maximum activity | Cupriavidus necator |
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